Label-free detection of low-molecular-weight samples using a terahertz chemical microscope

Takuya Kuwana, Masahiro Ogawa, Kenji Sakai, Toshihiko Kiwa, Keiji Tsukada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A terahertz chemical microscope (TCM) has been proposed and developed to visualize the distribution of biomolecular interactions on a sensing plate without any labels. In this study, the concanavalin A (Con A)-D-(+)-mannose (mannose) interaction was detected using the TCM with mannose applied as the analyte and Con A immobilized on the sensing plate. To demonstrate this interaction, the amplitude of terahertz pulses as a function of Con A-mannose interaction time, as well as the Con A-mannose coupling concentration, was evaluated. The results suggest that coupling kinetics may be evaluated using a TCM.

Original languageEnglish
Pages (from-to)42401
JournalApplied Physics Express
Volume9
Issue number4
DOIs
Publication statusPublished - Mar 14 2016

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low molecular weights
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Microscopes
Molecular weight
microscopes
interactions
Kinetics
kinetics
pulses

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Label-free detection of low-molecular-weight samples using a terahertz chemical microscope. / Kuwana, Takuya; Ogawa, Masahiro; Sakai, Kenji; Kiwa, Toshihiko; Tsukada, Keiji.

In: Applied Physics Express, Vol. 9, No. 4, 14.03.2016, p. 42401.

Research output: Contribution to journalArticle

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