L-Arginine administration attenuates airway inflammation by altering L-arginine metabolism in an NC/Nga mouse model of asthma

Ran Zhang, Masayuki Kubo, Ikuo Murakami, Heri Setiawan, Kei Takemoto, Kiyomi Inoue, Yoshihisa Fujikura, Keiki Ogino

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Changes in L-arginine metabolism, including increased arginase levels and decreased nitric oxide production, are involved in the pathophysiology of asthma. In this study, using an intranasal mite-induced NC/Nga mouse model of asthma, we examined whether administration of L-arginine ameliorated airway hyper responsiveness and inflammation by altering L-arginine metabolism. Experimental asthma was induced in NC/Nga mice via intranasal administration of mite crude extract (50 μg/day) on 5 consecutive days (days 0-4, sensitization) and on day 11 (challenge). Oral administration of L-arginine (250 mg/kg) was performed twice daily on days 5-10 for prevention or on days 11-13 for therapy. On day 14, we evaluated the inflammatory airway response (airway hyperresponsiveness, the number of cells in the bronchoalveolar lavage fluid, and the changes in pathological inflammation of the lung), arginase expression and activity, L-arginine bioavailability, and the concentration of NOx, the end products of nitric oxide. Treatment with L-arginine ameliorated the mite-induced inflammatory airway response. Furthermore, L- arginine administration attenuated the increases in arginase expression and activity and elevated the NOx levels by enhancing L-arginine bioavailability. These findings indicate that L-arginine administration may contribute to the improvement of asthmatic symptoms by altering L-arginine metabolism.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalJournal of Clinical Biochemistry and Nutrition
Volume56
Issue number3
DOIs
Publication statusPublished - May 1 2015

Fingerprint

Metabolism
Arginine
Asthma
Inflammation
Arginase
Mites
Biological Availability
Respiratory Hypersensitivity
Nitric Oxide
Intranasal Administration
Bronchoalveolar Lavage Fluid
Complex Mixtures
Oral Administration
Pneumonia
Cell Count
Fluids
Therapeutics

Keywords

  • Arginase
  • Asthma
  • L-arginine
  • L-arginine paradox
  • Nitric oxide

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

L-Arginine administration attenuates airway inflammation by altering L-arginine metabolism in an NC/Nga mouse model of asthma. / Zhang, Ran; Kubo, Masayuki; Murakami, Ikuo; Setiawan, Heri; Takemoto, Kei; Inoue, Kiyomi; Fujikura, Yoshihisa; Ogino, Keiki.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 56, No. 3, 01.05.2015, p. 201-207.

Research output: Contribution to journalArticle

Zhang, Ran ; Kubo, Masayuki ; Murakami, Ikuo ; Setiawan, Heri ; Takemoto, Kei ; Inoue, Kiyomi ; Fujikura, Yoshihisa ; Ogino, Keiki. / L-Arginine administration attenuates airway inflammation by altering L-arginine metabolism in an NC/Nga mouse model of asthma. In: Journal of Clinical Biochemistry and Nutrition. 2015 ; Vol. 56, No. 3. pp. 201-207.
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