l-2,4-diaminobutyric acid decarboxylase activity responsible for the formation of 1,3-diaminopropane in Enterobacter aerogenes

Hiroshi Nakao, Keiko Takeuchi, Sumio Shinoda, Shigeo Yamamoto

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

High content of l-2,4-diaminopropane (DAP), a normally minor derivative of polyamine metabolism, have been observed in cells of Enterobacter aerogenes. Supplementation of the growth medium with l-2,4-diaminobutyric acid (L-DABA) resulted in increased production of DAP, but not if supplemented with spermidine. On the basis of these observations, the biosynthetic route for DAP was evaluated. It has appeared that this bacterium possesses a novel enzyme activity catalysing the formation of DAP from L-DABA. Lack of the activity for oxidative cleavage of spermidine yielding DAP suggests that the enzyme termed DABA decarboxylase in responsible for the formation of DAP in this bacterium. The enzyme was partially purified 360-fold and some properties were examined. The pH optimum for the activity was 7.75-8.0, and the enzyme showed an absolute requirement for pyridoxal 5′-phosphate with the Km value of 41 μM. The Km value for L-DABA was 0.32 mM, and neither l-2,4-diaminopropionic acid, l-ornithine nor l-lysine showed detectable substrate activity towards the partially purified enzyme. Mg2+ and dithiothreitol greatly activated the enzyme.

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalFEMS Microbiology Letters
Volume70
Issue number1
DOIs
Publication statusPublished - Jun 15 1990

Fingerprint

Enterobacter aerogenes
Enzymes
Spermidine
Bacteria
Pyridoxal Phosphate
Ornithine
Carboxy-Lyases
Dithiothreitol
Polyamines
Lysine
2,4-diaminobutyric acid decarboxylase
trimethylenediamine
Acids
Growth
2,4-diaminobutyric acid

Keywords

  • Decarboxylase
  • Diamine
  • Enterobacter
  • Polyamine distribution

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

l-2,4-diaminobutyric acid decarboxylase activity responsible for the formation of 1,3-diaminopropane in Enterobacter aerogenes. / Nakao, Hiroshi; Takeuchi, Keiko; Shinoda, Sumio; Yamamoto, Shigeo.

In: FEMS Microbiology Letters, Vol. 70, No. 1, 15.06.1990, p. 61-66.

Research output: Contribution to journalArticle

Nakao, Hiroshi ; Takeuchi, Keiko ; Shinoda, Sumio ; Yamamoto, Shigeo. / l-2,4-diaminobutyric acid decarboxylase activity responsible for the formation of 1,3-diaminopropane in Enterobacter aerogenes. In: FEMS Microbiology Letters. 1990 ; Vol. 70, No. 1. pp. 61-66.
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