Kv1.2-immunoreactive primary sensory neurons in the rat trigeminal ganglion

Hiroyuki Ichikawa, Tomosada Sugimoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Immunohistochemistry for Kv1.2, a subunit of voltage-gated K+ channels, was performed on the trigeminal ganglion (TG). Immunoreactivity (ir) was detected in half (48%) the TG neurons. These neurons were mostly medium-sized to large (range 137.6-2664.8 μm2, mean±S.D. 892.6±413.3 μm2). A double immunofluorescence method also revealed co-expression of Kv1.2 and parvalbumin. Half (54%) the Kv1.2-immunoreactive (ir) neurons exhibited parvalbumin-ir, and parvalbumin-ir neurons mostly showed Kv1.2-ir (95%). Kv1.2-ir neurons which co-expressed CGRP-ir were rare in this ganglion. Some 40% of TG neurons retrogradely labeled from the facial skin exhibited Kv1.2-ir, whereas ir was detected in 16% of those labeled from the tooth pulp. The present study indicates that Kv1.2-ir TG neurons include low-threshold mechanoreceptors and nociceptors which innervate the facial skin and tooth pulp, respectively.

Original languageEnglish
Pages (from-to)222-227
Number of pages6
JournalBrain Research
Volume974
Issue number1-2
DOIs
Publication statusPublished - Jun 6 2003

Fingerprint

Trigeminal Ganglion
Sensory Receptor Cells
Neurons
Parvalbumins
Tooth
Voltage-Gated Potassium Channels
Skin
Nociceptors
Mechanoreceptors
Ganglia
Fluorescent Antibody Technique
Immunohistochemistry

Keywords

  • Calcitonin gene-related peptide
  • Facial skin
  • Immunohistochemistry
  • Kv1.2
  • Parvalbumin
  • Tooth pulp
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kv1.2-immunoreactive primary sensory neurons in the rat trigeminal ganglion. / Ichikawa, Hiroyuki; Sugimoto, Tomosada.

In: Brain Research, Vol. 974, No. 1-2, 06.06.2003, p. 222-227.

Research output: Contribution to journalArticle

Ichikawa, Hiroyuki ; Sugimoto, Tomosada. / Kv1.2-immunoreactive primary sensory neurons in the rat trigeminal ganglion. In: Brain Research. 2003 ; Vol. 974, No. 1-2. pp. 222-227.
@article{94baa2ed5c1a439889fb9265ce4aaf4e,
title = "Kv1.2-immunoreactive primary sensory neurons in the rat trigeminal ganglion",
abstract = "Immunohistochemistry for Kv1.2, a subunit of voltage-gated K+ channels, was performed on the trigeminal ganglion (TG). Immunoreactivity (ir) was detected in half (48{\%}) the TG neurons. These neurons were mostly medium-sized to large (range 137.6-2664.8 μm2, mean±S.D. 892.6±413.3 μm2). A double immunofluorescence method also revealed co-expression of Kv1.2 and parvalbumin. Half (54{\%}) the Kv1.2-immunoreactive (ir) neurons exhibited parvalbumin-ir, and parvalbumin-ir neurons mostly showed Kv1.2-ir (95{\%}). Kv1.2-ir neurons which co-expressed CGRP-ir were rare in this ganglion. Some 40{\%} of TG neurons retrogradely labeled from the facial skin exhibited Kv1.2-ir, whereas ir was detected in 16{\%} of those labeled from the tooth pulp. The present study indicates that Kv1.2-ir TG neurons include low-threshold mechanoreceptors and nociceptors which innervate the facial skin and tooth pulp, respectively.",
keywords = "Calcitonin gene-related peptide, Facial skin, Immunohistochemistry, Kv1.2, Parvalbumin, Tooth pulp, Trigeminal ganglion",
author = "Hiroyuki Ichikawa and Tomosada Sugimoto",
year = "2003",
month = "6",
day = "6",
doi = "10.1016/S0006-8993(03)02583-6",
language = "English",
volume = "974",
pages = "222--227",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Kv1.2-immunoreactive primary sensory neurons in the rat trigeminal ganglion

AU - Ichikawa, Hiroyuki

AU - Sugimoto, Tomosada

PY - 2003/6/6

Y1 - 2003/6/6

N2 - Immunohistochemistry for Kv1.2, a subunit of voltage-gated K+ channels, was performed on the trigeminal ganglion (TG). Immunoreactivity (ir) was detected in half (48%) the TG neurons. These neurons were mostly medium-sized to large (range 137.6-2664.8 μm2, mean±S.D. 892.6±413.3 μm2). A double immunofluorescence method also revealed co-expression of Kv1.2 and parvalbumin. Half (54%) the Kv1.2-immunoreactive (ir) neurons exhibited parvalbumin-ir, and parvalbumin-ir neurons mostly showed Kv1.2-ir (95%). Kv1.2-ir neurons which co-expressed CGRP-ir were rare in this ganglion. Some 40% of TG neurons retrogradely labeled from the facial skin exhibited Kv1.2-ir, whereas ir was detected in 16% of those labeled from the tooth pulp. The present study indicates that Kv1.2-ir TG neurons include low-threshold mechanoreceptors and nociceptors which innervate the facial skin and tooth pulp, respectively.

AB - Immunohistochemistry for Kv1.2, a subunit of voltage-gated K+ channels, was performed on the trigeminal ganglion (TG). Immunoreactivity (ir) was detected in half (48%) the TG neurons. These neurons were mostly medium-sized to large (range 137.6-2664.8 μm2, mean±S.D. 892.6±413.3 μm2). A double immunofluorescence method also revealed co-expression of Kv1.2 and parvalbumin. Half (54%) the Kv1.2-immunoreactive (ir) neurons exhibited parvalbumin-ir, and parvalbumin-ir neurons mostly showed Kv1.2-ir (95%). Kv1.2-ir neurons which co-expressed CGRP-ir were rare in this ganglion. Some 40% of TG neurons retrogradely labeled from the facial skin exhibited Kv1.2-ir, whereas ir was detected in 16% of those labeled from the tooth pulp. The present study indicates that Kv1.2-ir TG neurons include low-threshold mechanoreceptors and nociceptors which innervate the facial skin and tooth pulp, respectively.

KW - Calcitonin gene-related peptide

KW - Facial skin

KW - Immunohistochemistry

KW - Kv1.2

KW - Parvalbumin

KW - Tooth pulp

KW - Trigeminal ganglion

UR - http://www.scopus.com/inward/record.url?scp=0037651089&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037651089&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(03)02583-6

DO - 10.1016/S0006-8993(03)02583-6

M3 - Article

C2 - 12742640

AN - SCOPUS:0037651089

VL - 974

SP - 222

EP - 227

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -