Kupffer cell function in chronic liver injury and after partial hepatectomy

Keisuke Hamazaki, Shizo Sato, Masayuki Yunoki, Takuo Noda, Luis Fernando Moreira, Hisashi Mimura, Kunzo Orita

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The reticuloendothelial system (RES) plays an important role in the biological defense system. In the liver, Kupffer cells are the main constituent of the RES, and when their function is impaired postoperative complications may more often occur. By using99mTc-labeled human serum albumin millimicrospheres (99mTc-HSA-MM) combined with assessment of single photon emission computed tomography (SPECT), we have attempted to determine the function of Kupffer cells independently of the hepatic blood flow. First, Kupffer cell function in rats with chronic liver injury caused by CCl4 was studied. The hepatic uptake rate in chronic liver injury was decreased, and a reduced phagocytic activity of the Kupffer cells was noted. The parameter concerning Kupffer cell degradation, the excretion rate (k), was markedly decreased in the early period of chronic liver injury. Changes in Kupffer cell function after 30% and 70% hepatectomy were also studied. After 30% hepatectomy, the excretion rate was decreased on the first postoperative day (POD), and it was increased beyond that found after sham operation on the 3rd POD. In contrast, slower recovery of uptake rate was demonstrated. After 70% hepatectomy, both uptake and excretion rates were markedly reduced, and recovery was prolonged beyond the 5th POD. The hepatic uptake was not parallel with the excretion rate in either experiment. These results suggest that the method that measures the hepatic excretion rate may provide a better assessment of Kupffer cell function than the current uptake measurement with radiolabeled colloid.

Original languageEnglish
Pages (from-to)237-246
Number of pages10
JournalResearch in Experimental Medicine
Volume194
Issue number1
DOIs
Publication statusPublished - Dec 1994

Fingerprint

Kupffer Cells
Hepatectomy
Liver
Wounds and Injuries
Mononuclear Phagocyte System
Technetium Tc 99m Aggregated Albumin
Colloids
Single-Photon Emission-Computed Tomography
Serum Albumin

Keywords

  • Hepatectomy
  • Kupffer cell
  • Liver injury

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hamazaki, K., Sato, S., Yunoki, M., Noda, T., Moreira, L. F., Mimura, H., & Orita, K. (1994). Kupffer cell function in chronic liver injury and after partial hepatectomy. Research in Experimental Medicine, 194(1), 237-246. https://doi.org/10.1007/BF02576385

Kupffer cell function in chronic liver injury and after partial hepatectomy. / Hamazaki, Keisuke; Sato, Shizo; Yunoki, Masayuki; Noda, Takuo; Moreira, Luis Fernando; Mimura, Hisashi; Orita, Kunzo.

In: Research in Experimental Medicine, Vol. 194, No. 1, 12.1994, p. 237-246.

Research output: Contribution to journalArticle

Hamazaki, K, Sato, S, Yunoki, M, Noda, T, Moreira, LF, Mimura, H & Orita, K 1994, 'Kupffer cell function in chronic liver injury and after partial hepatectomy', Research in Experimental Medicine, vol. 194, no. 1, pp. 237-246. https://doi.org/10.1007/BF02576385
Hamazaki, Keisuke ; Sato, Shizo ; Yunoki, Masayuki ; Noda, Takuo ; Moreira, Luis Fernando ; Mimura, Hisashi ; Orita, Kunzo. / Kupffer cell function in chronic liver injury and after partial hepatectomy. In: Research in Experimental Medicine. 1994 ; Vol. 194, No. 1. pp. 237-246.
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