Kupffer cell activation in the survival discrepancy between liver grafts from enterally and parenterally fed donors

T. Ishikawa, Takahito Yagi, H. Sadamori, N. Ishine, H. Sasaki, M. Oishi, N. Tanaka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study was designed to investigate the effects of differences in the route of nutritional support of the donor on cold ischemia/reperfusion injury. Participation of Kupffer cells in these effects, based on the analysis of hepatic energy metabolism in early phases of reperfusion was also investigated. Orthotopic liver transplantation was performed between Large-White pigs weighing 20-30 kg after a 4-h cold preservation of the graft in Euro-Collins solution at 4°C. One group was fed orally with a standard laboratory diet (FED group, n = 5), a second group was fasted and given 20% glucose intravenously (12 kJ/kg per day) (PEF group, n = 5), and a third group was fed orally with a standard laboratory diet and given GdCl3 (10 mg/kg) intravenously 24 h before operation (FEDGD group, n = 5). These treatments were given for 7 days prior to harvesting. The survival time was significantly longer in the PEF (34.8 ± 5.5 days) and FEDGD (28.0 ± 11.9 days) groups than in the FED (9.8 ± 2.0 days) group (P <0.05). The serum hyaluronic acid elimination rate determined from 1 to 2 h after reperfusion was significantly lower in the FED group than in the other two groups (P <0.001). The glycogen content of the livers l h after reperfusion in all three groups had been consumed rapidly, but the ATP content of the livers was significantly reduced in the FED group alone (P <0.01). Hepatic FFA clearance (CFFA) was moderately increased in all three groups in the early phase after reperfusion, but it was higher in the FED group than in the other two groups, with significant differences 1 and 2 h after reperfusion (P <0.05). In conclusion, parenteral nutrition of the donors reduced cold ischemia/ reperfusion injury which is related to Kupffer cell activation and, thus, was better than enteral nutrition for donor management.

Original languageEnglish
JournalTransplant international : official journal of the European Society for Organ Transplantation
Volume11
Issue numberSUPPL. 1
Publication statusPublished - 1998

Fingerprint

Kupffer Cells
Reperfusion
Transplants
Liver
Cold Ischemia
Reperfusion Injury
Diet
Liver Glycogen
Nutritional Support
Parenteral Nutrition
Enteral Nutrition
Hyaluronic Acid
Liver Transplantation
Energy Metabolism
Swine
Adenosine Triphosphate
Glucose
Serum

Keywords

  • Energy metabolism
  • Free fatty acid
  • Glycogen
  • Kupffer cell
  • Liver transplantation

ASJC Scopus subject areas

  • Transplantation

Cite this

Kupffer cell activation in the survival discrepancy between liver grafts from enterally and parenterally fed donors. / Ishikawa, T.; Yagi, Takahito; Sadamori, H.; Ishine, N.; Sasaki, H.; Oishi, M.; Tanaka, N.

In: Transplant international : official journal of the European Society for Organ Transplantation, Vol. 11, No. SUPPL. 1, 1998.

Research output: Contribution to journalArticle

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abstract = "This study was designed to investigate the effects of differences in the route of nutritional support of the donor on cold ischemia/reperfusion injury. Participation of Kupffer cells in these effects, based on the analysis of hepatic energy metabolism in early phases of reperfusion was also investigated. Orthotopic liver transplantation was performed between Large-White pigs weighing 20-30 kg after a 4-h cold preservation of the graft in Euro-Collins solution at 4°C. One group was fed orally with a standard laboratory diet (FED group, n = 5), a second group was fasted and given 20{\%} glucose intravenously (12 kJ/kg per day) (PEF group, n = 5), and a third group was fed orally with a standard laboratory diet and given GdCl3 (10 mg/kg) intravenously 24 h before operation (FEDGD group, n = 5). These treatments were given for 7 days prior to harvesting. The survival time was significantly longer in the PEF (34.8 ± 5.5 days) and FEDGD (28.0 ± 11.9 days) groups than in the FED (9.8 ± 2.0 days) group (P <0.05). The serum hyaluronic acid elimination rate determined from 1 to 2 h after reperfusion was significantly lower in the FED group than in the other two groups (P <0.001). The glycogen content of the livers l h after reperfusion in all three groups had been consumed rapidly, but the ATP content of the livers was significantly reduced in the FED group alone (P <0.01). Hepatic FFA clearance (CFFA) was moderately increased in all three groups in the early phase after reperfusion, but it was higher in the FED group than in the other two groups, with significant differences 1 and 2 h after reperfusion (P <0.05). In conclusion, parenteral nutrition of the donors reduced cold ischemia/ reperfusion injury which is related to Kupffer cell activation and, thus, was better than enteral nutrition for donor management.",
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AU - Ishikawa, T.

AU - Yagi, Takahito

AU - Sadamori, H.

AU - Ishine, N.

AU - Sasaki, H.

AU - Oishi, M.

AU - Tanaka, N.

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N2 - This study was designed to investigate the effects of differences in the route of nutritional support of the donor on cold ischemia/reperfusion injury. Participation of Kupffer cells in these effects, based on the analysis of hepatic energy metabolism in early phases of reperfusion was also investigated. Orthotopic liver transplantation was performed between Large-White pigs weighing 20-30 kg after a 4-h cold preservation of the graft in Euro-Collins solution at 4°C. One group was fed orally with a standard laboratory diet (FED group, n = 5), a second group was fasted and given 20% glucose intravenously (12 kJ/kg per day) (PEF group, n = 5), and a third group was fed orally with a standard laboratory diet and given GdCl3 (10 mg/kg) intravenously 24 h before operation (FEDGD group, n = 5). These treatments were given for 7 days prior to harvesting. The survival time was significantly longer in the PEF (34.8 ± 5.5 days) and FEDGD (28.0 ± 11.9 days) groups than in the FED (9.8 ± 2.0 days) group (P <0.05). The serum hyaluronic acid elimination rate determined from 1 to 2 h after reperfusion was significantly lower in the FED group than in the other two groups (P <0.001). The glycogen content of the livers l h after reperfusion in all three groups had been consumed rapidly, but the ATP content of the livers was significantly reduced in the FED group alone (P <0.01). Hepatic FFA clearance (CFFA) was moderately increased in all three groups in the early phase after reperfusion, but it was higher in the FED group than in the other two groups, with significant differences 1 and 2 h after reperfusion (P <0.05). In conclusion, parenteral nutrition of the donors reduced cold ischemia/ reperfusion injury which is related to Kupffer cell activation and, thus, was better than enteral nutrition for donor management.

AB - This study was designed to investigate the effects of differences in the route of nutritional support of the donor on cold ischemia/reperfusion injury. Participation of Kupffer cells in these effects, based on the analysis of hepatic energy metabolism in early phases of reperfusion was also investigated. Orthotopic liver transplantation was performed between Large-White pigs weighing 20-30 kg after a 4-h cold preservation of the graft in Euro-Collins solution at 4°C. One group was fed orally with a standard laboratory diet (FED group, n = 5), a second group was fasted and given 20% glucose intravenously (12 kJ/kg per day) (PEF group, n = 5), and a third group was fed orally with a standard laboratory diet and given GdCl3 (10 mg/kg) intravenously 24 h before operation (FEDGD group, n = 5). These treatments were given for 7 days prior to harvesting. The survival time was significantly longer in the PEF (34.8 ± 5.5 days) and FEDGD (28.0 ± 11.9 days) groups than in the FED (9.8 ± 2.0 days) group (P <0.05). The serum hyaluronic acid elimination rate determined from 1 to 2 h after reperfusion was significantly lower in the FED group than in the other two groups (P <0.001). The glycogen content of the livers l h after reperfusion in all three groups had been consumed rapidly, but the ATP content of the livers was significantly reduced in the FED group alone (P <0.01). Hepatic FFA clearance (CFFA) was moderately increased in all three groups in the early phase after reperfusion, but it was higher in the FED group than in the other two groups, with significant differences 1 and 2 h after reperfusion (P <0.05). In conclusion, parenteral nutrition of the donors reduced cold ischemia/ reperfusion injury which is related to Kupffer cell activation and, thus, was better than enteral nutrition for donor management.

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