Konjak mosaic virus: The complete nucleotide sequence of the genomic RNA and its comparison with other potyviruses

M. Nishiguchi, S. Yamasaki, X. Z. Lu, A. Shimoyama, K. Hanada, S. Sonoda, M. Shimono, J. Sakai, Y. Mikoshiba, I. Fujisawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Konjak mosaic virus (KoMV) belongs to the genus Potyvirus, family Potyviridae. The complete nucleotide sequence of KoMV F isolate (KoMV F) was determined. The genome is 9,544 nucleotides long excluding the 3′ terminal poly A tail and encodes a typical potyviral 350-kDa polyprotein of 3,087 amino acids. Phylogenetic analysis using known potyvirus polyproteins shows that KoMV constitutes a branch with yam mosaic virus, close to another branch including Japanese yam mosaic virus, turnip mosaic virus, scallion mosaic virus and lettuce mosaic virus. The 3′ terminal 1,842 nucleotides of a different isolate of KoMV, K-2, was also determined, covering the C-terminal 292 amino acids of the nuclear inclusion protein b (NIb), coat protein (CP), and the 3′ untranslated region. The amino acid sequences of the KoMV F CP and the nucleotide sequences of the KoMV F 3′ untranslated region showed 92.5 and 90.5% identity to the corresponding genes of K-2, 88.7-96.8 and 92.7-94.4% to those of Zantedeschia mosaic virus (ZaMV) isolates, 87.5-89.7% and 85.5-90.3% to those of Japanese hornwort mosaic virus (JHMV) isolates. These results showed that KoMV is a distinct potyvirus and that KoMV, ZaMV, and JHMV are members of the same potyvirus species. Considering that KoMV was the first of these to be described, ZaMV and JHMV may be considered isolates of KoMV.

Original languageEnglish
Pages (from-to)1643-1650
Number of pages8
JournalArchives of Virology
Volume151
Issue number8
DOIs
Publication statusPublished - Aug 2006
Externally publishedYes

Fingerprint

Potyvirus
Mosaic Viruses
RNA
Zantedeschia
Anthocerotophyta
Dioscorea
Polyproteins
Capsid Proteins
3' Untranslated Regions
Potyviridae
Nucleotides

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Nishiguchi, M., Yamasaki, S., Lu, X. Z., Shimoyama, A., Hanada, K., Sonoda, S., ... Fujisawa, I. (2006). Konjak mosaic virus: The complete nucleotide sequence of the genomic RNA and its comparison with other potyviruses. Archives of Virology, 151(8), 1643-1650. https://doi.org/10.1007/s00705-006-0735-x

Konjak mosaic virus : The complete nucleotide sequence of the genomic RNA and its comparison with other potyviruses. / Nishiguchi, M.; Yamasaki, S.; Lu, X. Z.; Shimoyama, A.; Hanada, K.; Sonoda, S.; Shimono, M.; Sakai, J.; Mikoshiba, Y.; Fujisawa, I.

In: Archives of Virology, Vol. 151, No. 8, 08.2006, p. 1643-1650.

Research output: Contribution to journalArticle

Nishiguchi, M, Yamasaki, S, Lu, XZ, Shimoyama, A, Hanada, K, Sonoda, S, Shimono, M, Sakai, J, Mikoshiba, Y & Fujisawa, I 2006, 'Konjak mosaic virus: The complete nucleotide sequence of the genomic RNA and its comparison with other potyviruses', Archives of Virology, vol. 151, no. 8, pp. 1643-1650. https://doi.org/10.1007/s00705-006-0735-x
Nishiguchi, M. ; Yamasaki, S. ; Lu, X. Z. ; Shimoyama, A. ; Hanada, K. ; Sonoda, S. ; Shimono, M. ; Sakai, J. ; Mikoshiba, Y. ; Fujisawa, I. / Konjak mosaic virus : The complete nucleotide sequence of the genomic RNA and its comparison with other potyviruses. In: Archives of Virology. 2006 ; Vol. 151, No. 8. pp. 1643-1650.
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