Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher

Yasuhiro Kambayashi, Susumu Takekoshi, Minoru Nakano, Masafumi Shibamori, Yoshiaki Hitomi, Keiki Ogino

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We recently reported that kinobeon A, produced from safflower cells, suppressed the free radical-induced damage of cell and microsomal membranes. In the present study, we investigated whether kinobeon A quenches singlet oxygen, another important active oxygen species. Kinobeon A inhibited the singlet oxygen-induced oxidation of squalene. The second-order rate constant between singlet oxygen and kinobeon A was 1.15 × 1010 M -1s-1 in methanol containing 10% dimethyl sulfoxide at 37°C. Those of α-tocopherol and β-carotene, which are known potent singlet oxygen quenchers, were 4.45 × 108 M-1s-1 and 1.26 × 1010 M -1s-1, respectively. When kinobeon A was incubated with a thermolytic singlet oxygen generator, its concentration decreased. However, this change was extremely small compared to the amount of singlet oxygen formed and the inhibitory effect of kinobeon A on squalene oxidation by singlet oxygen. In conclusion, kinobeon A was a strong singlet oxygen quencher. It reacted chemically with singlet oxygen, but it was physical quenching that was mainly responsible for the elimination of singlet oxygen by kinobeon A. Kinobeon A is expected to have a preventive e.ect on singlet oxygen-related diseases of the skin or eyes.

Original languageEnglish
Pages (from-to)903-907
Number of pages5
JournalActa Biochimica Polonica
Volume52
Issue number4
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Carthamus tinctorius
Singlet Oxygen
Cultured Cells
Squalene
kinobeon A
Oxidation
Tocopherols
Eye Diseases
Carotenoids
Dimethyl Sulfoxide
Skin Diseases
Free Radicals
Methanol
Quenching
Rate constants
Reactive Oxygen Species
Skin

Keywords

  • Antioxidant
  • Endoperoxide
  • Kinobeon A
  • Quencher
  • Safflower
  • Singlet oxygen

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Kambayashi, Y., Takekoshi, S., Nakano, M., Shibamori, M., Hitomi, Y., & Ogino, K. (2005). Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher. Acta Biochimica Polonica, 52(4), 903-907.

Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher. / Kambayashi, Yasuhiro; Takekoshi, Susumu; Nakano, Minoru; Shibamori, Masafumi; Hitomi, Yoshiaki; Ogino, Keiki.

In: Acta Biochimica Polonica, Vol. 52, No. 4, 2005, p. 903-907.

Research output: Contribution to journalArticle

Kambayashi, Y, Takekoshi, S, Nakano, M, Shibamori, M, Hitomi, Y & Ogino, K 2005, 'Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher', Acta Biochimica Polonica, vol. 52, no. 4, pp. 903-907.
Kambayashi Y, Takekoshi S, Nakano M, Shibamori M, Hitomi Y, Ogino K. Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher. Acta Biochimica Polonica. 2005;52(4):903-907.
Kambayashi, Yasuhiro ; Takekoshi, Susumu ; Nakano, Minoru ; Shibamori, Masafumi ; Hitomi, Yoshiaki ; Ogino, Keiki. / Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher. In: Acta Biochimica Polonica. 2005 ; Vol. 52, No. 4. pp. 903-907.
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