TY - JOUR
T1 - Kinetics of expression of connective tissue growth factor gene during liver regeneration after partial hepatectomy and D-galactosamine-induced liver injury in rats
AU - Ujike, Kozo
AU - Shinji, Toshiyuki
AU - Hirasaki, Shoji
AU - Shiraha, Hidenori
AU - Nakamura, Masaki
AU - Tsuji, Takao
AU - Koide, Norio
N1 - Funding Information:
We thank Professor Takashi Muramatsu, Department of Biochemistry, Faculty of Medicine, Nagoya University, for criticism of the manuscript. This work was supported in part by a Grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture.
PY - 2000/10/22
Y1 - 2000/10/22
N2 - Connective tissue growth factor (CTGF) is up-regulated by TGF-β1 during wound healing. The present study examined the expression of CTGF during regeneration after 70% partial hepatectomy (PH) or D-galactosamine (GalN)-injured liver in rats. CTGF, TGF-β1, and type I collagen mRNAs were semiquantified by a ribonuclease protection assay. After PH, TGF-β1 and type I collagen were increased at 2-6 h and at 12-48 h. CTGF increased at 6 h and returned to the control level thereafter. The ribonuclease protection assay of cultured hepatic stellate cells (HSC) and in situ hybridization suggest that the cells express CTGF along sinusoid might be HSCs. After GalN administration, CTGF increased at 2-96 h with a shoulder peak at 6-12 h followed by a main peak at 24 h. TGF-β1 and type I collagen were up-regulated with kinetics similar to those of CTGF. The different kinetics between PH and GalN regenerations indicate that regulation of CTGF in the two processes is different. Higher TGF-β1 expression after inflammatory/necrotic process in the GalN regeneration may caused the prolonged CTGF expression. (C) 2000 Academic Press.
AB - Connective tissue growth factor (CTGF) is up-regulated by TGF-β1 during wound healing. The present study examined the expression of CTGF during regeneration after 70% partial hepatectomy (PH) or D-galactosamine (GalN)-injured liver in rats. CTGF, TGF-β1, and type I collagen mRNAs were semiquantified by a ribonuclease protection assay. After PH, TGF-β1 and type I collagen were increased at 2-6 h and at 12-48 h. CTGF increased at 6 h and returned to the control level thereafter. The ribonuclease protection assay of cultured hepatic stellate cells (HSC) and in situ hybridization suggest that the cells express CTGF along sinusoid might be HSCs. After GalN administration, CTGF increased at 2-96 h with a shoulder peak at 6-12 h followed by a main peak at 24 h. TGF-β1 and type I collagen were up-regulated with kinetics similar to those of CTGF. The different kinetics between PH and GalN regenerations indicate that regulation of CTGF in the two processes is different. Higher TGF-β1 expression after inflammatory/necrotic process in the GalN regeneration may caused the prolonged CTGF expression. (C) 2000 Academic Press.
KW - Cell cycle
KW - Collagen
KW - Connective tissue growth factor
KW - D-galactosamine
KW - Extracellular matrix
KW - Hepatic stellate cell
KW - In situ hybridization
KW - Partial hepatectomy
KW - Transforming growth factor beta
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U2 - 10.1006/bbrc.2000.3693
DO - 10.1006/bbrc.2000.3693
M3 - Article
C2 - 11032743
AN - SCOPUS:0034703632
VL - 277
SP - 448
EP - 454
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -