Kinetic study on the reversible hydride transfer between methylene blue and thionine

Y. Liu, S. Yamamoto, Y. Fujiyama, Y. Sueishi

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11 Citations (Scopus)

Abstract

The kinetics of the hydride transfer reaction between leuco methylene blue and thionine was investigated spectrophotometrically by means of the stopped-flow technique. Leuco methylene blue and leuco thionine were produced by photoreduction of methylene blue and thionine with triethylamine in ethanol in the drive syringe of the stopped-flow apparatus and their solutions were used as such in the measurement of the reaction rate. This reaction was found to be reversible, and the rate constants for forward and backward reactions were obtained. The ratio of k(f)/k(b) is consistent with the value of the equilibrium constant (K) which was obtained from the absorbance at 655 nm of methylene blue at equilibrium using the stoichiometric relationships and conservation conditions. The mechanism of this reversible hydride transfer reaction was discussed.

Original languageEnglish
Pages (from-to)2367-2371
Number of pages5
JournalPhysical Chemistry Chemical Physics
Volume2
Issue number10
DOIs
Publication statusPublished - May 15 2000

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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