Kinetic study on giant vesicle formation with electroformation method

Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Giant vesicles (GVs) composed of zwitterionic phospholipids were prepared by the electroformation method. The growth behavior of GVs was quantitatively analyzed as a first-order kinetics of the radius of GVs to obtain the apparent growth rate constant kGr. On the basis of the dependence of the kGr value on the preparation temperature and the lipid composition of GVs, the membrane fluidity of vesicle membranes was found to be dominant in the growth behavior of GVs. The comparison of the kGr values with the membrane fluidity for GVs suggested the validity of the model that the swelling of the lipid membrane could induce the growth of GVs.

Original languageEnglish
Pages (from-to)4835-4840
Number of pages6
JournalLangmuir
Volume25
Issue number9
DOIs
Publication statusPublished - May 5 2009
Externally publishedYes

Fingerprint

Fluidity
membranes
Membranes
Kinetics
kinetics
lipids
Phospholipids
Membrane Lipids
Lipids
Swelling
Rate constants
swelling
Chemical analysis
preparation
radii
Temperature
temperature

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Kinetic study on giant vesicle formation with electroformation method. / Shimanouchi, Toshinori; Umakoshi, Hiroshi; Kuboi, Ryoichi.

In: Langmuir, Vol. 25, No. 9, 05.05.2009, p. 4835-4840.

Research output: Contribution to journalArticle

Shimanouchi, Toshinori ; Umakoshi, Hiroshi ; Kuboi, Ryoichi. / Kinetic study on giant vesicle formation with electroformation method. In: Langmuir. 2009 ; Vol. 25, No. 9. pp. 4835-4840.
@article{0576dc886354491f9559f3209f43ee46,
title = "Kinetic study on giant vesicle formation with electroformation method",
abstract = "Giant vesicles (GVs) composed of zwitterionic phospholipids were prepared by the electroformation method. The growth behavior of GVs was quantitatively analyzed as a first-order kinetics of the radius of GVs to obtain the apparent growth rate constant kGr. On the basis of the dependence of the kGr value on the preparation temperature and the lipid composition of GVs, the membrane fluidity of vesicle membranes was found to be dominant in the growth behavior of GVs. The comparison of the kGr values with the membrane fluidity for GVs suggested the validity of the model that the swelling of the lipid membrane could induce the growth of GVs.",
author = "Toshinori Shimanouchi and Hiroshi Umakoshi and Ryoichi Kuboi",
year = "2009",
month = "5",
day = "5",
doi = "10.1021/la8040488",
language = "English",
volume = "25",
pages = "4835--4840",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - Kinetic study on giant vesicle formation with electroformation method

AU - Shimanouchi, Toshinori

AU - Umakoshi, Hiroshi

AU - Kuboi, Ryoichi

PY - 2009/5/5

Y1 - 2009/5/5

N2 - Giant vesicles (GVs) composed of zwitterionic phospholipids were prepared by the electroformation method. The growth behavior of GVs was quantitatively analyzed as a first-order kinetics of the radius of GVs to obtain the apparent growth rate constant kGr. On the basis of the dependence of the kGr value on the preparation temperature and the lipid composition of GVs, the membrane fluidity of vesicle membranes was found to be dominant in the growth behavior of GVs. The comparison of the kGr values with the membrane fluidity for GVs suggested the validity of the model that the swelling of the lipid membrane could induce the growth of GVs.

AB - Giant vesicles (GVs) composed of zwitterionic phospholipids were prepared by the electroformation method. The growth behavior of GVs was quantitatively analyzed as a first-order kinetics of the radius of GVs to obtain the apparent growth rate constant kGr. On the basis of the dependence of the kGr value on the preparation temperature and the lipid composition of GVs, the membrane fluidity of vesicle membranes was found to be dominant in the growth behavior of GVs. The comparison of the kGr values with the membrane fluidity for GVs suggested the validity of the model that the swelling of the lipid membrane could induce the growth of GVs.

UR - http://www.scopus.com/inward/record.url?scp=66149090315&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66149090315&partnerID=8YFLogxK

U2 - 10.1021/la8040488

DO - 10.1021/la8040488

M3 - Article

C2 - 19334719

AN - SCOPUS:66149090315

VL - 25

SP - 4835

EP - 4840

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 9

ER -