Key cofactors of Photosystem II cores from four organisms identified by 1.7-K absorption, CD and MCD

Sindra Peterson Årsköld, Paul J. Smith, Jian-Ren Shen, Ron J. Pace, Elmars Krausz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Active Photosystem II (PS II) cores were prepared from spinach, pea, Synechocystis PCC 6803, and Thermosynechococcus vulcanus, the latter of which has been structurally determined [Kamiya and Shen (2003) Proc Natl Acad Sci USA 100: 98-103]. Electrochromic shifts resulting from QA reduction by 1.7-K illumination were recorded, and the Qx and Qy absorption bands of the redox-active pheophytin a thus identified in the different organisms. The Qx transition is ∼3 nm (100 cm -1) to higher energy in cyanobacteria than in the plants. The predominant Qy shift appears in the range 683-686 nm depending on species, and does not appear to have a systematic shift. Low-temperature absorption, circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of the chlorophyll Qy region are very similar in spinach and pea, but vary in cyanobacteria. We assigned CP43 and CP47 trap-chlorophyll absorption features in all species, as well as a P680 transition. Each absorption identified has an area of one chlorophyll a. The MCD deficit, introduced previously for spinach as an indicator of P680 activity, occurs in the same spectral region and has the same area in all species, pointing to a robustness of this as a signature for P680. MCD and CD characteristics point towards a significant variance in P680 structure between cyanobacteria, thermophilic cyanobacteria, and higher plants.

Original languageEnglish
Pages (from-to)309-316
Number of pages8
JournalPhotosynthesis Research
Volume84
Issue number1-3
DOIs
Publication statusPublished - Jun 2005

Fingerprint

circular dichroism spectroscopy
Photosystem II Protein Complex
Circular Dichroism
photosystem II
Cyanobacteria
Spinacia oleracea
spinach
organisms
Chlorophyll
Peas
chlorophyll
Thermosynechococcus vulcanus
peas
Synechocystis
Absorption spectra
Lighting
Oxidation-Reduction
lighting
traps
Temperature

Keywords

  • CD
  • Chlorophyll
  • Electrochromic shift
  • MCD
  • P680
  • Pea
  • Pheophytin
  • PS II
  • Spinach
  • Synechocystis PCC 6803
  • Thermosynechococcus vulcanus

ASJC Scopus subject areas

  • Plant Science

Cite this

Key cofactors of Photosystem II cores from four organisms identified by 1.7-K absorption, CD and MCD. / Peterson Årsköld, Sindra; Smith, Paul J.; Shen, Jian-Ren; Pace, Ron J.; Krausz, Elmars.

In: Photosynthesis Research, Vol. 84, No. 1-3, 06.2005, p. 309-316.

Research output: Contribution to journalArticle

Peterson Årsköld, Sindra ; Smith, Paul J. ; Shen, Jian-Ren ; Pace, Ron J. ; Krausz, Elmars. / Key cofactors of Photosystem II cores from four organisms identified by 1.7-K absorption, CD and MCD. In: Photosynthesis Research. 2005 ; Vol. 84, No. 1-3. pp. 309-316.
@article{0fc6a8c833354eeb905da9fcd2ddb6ed,
title = "Key cofactors of Photosystem II cores from four organisms identified by 1.7-K absorption, CD and MCD",
abstract = "Active Photosystem II (PS II) cores were prepared from spinach, pea, Synechocystis PCC 6803, and Thermosynechococcus vulcanus, the latter of which has been structurally determined [Kamiya and Shen (2003) Proc Natl Acad Sci USA 100: 98-103]. Electrochromic shifts resulting from QA reduction by 1.7-K illumination were recorded, and the Qx and Qy absorption bands of the redox-active pheophytin a thus identified in the different organisms. The Qx transition is ∼3 nm (100 cm -1) to higher energy in cyanobacteria than in the plants. The predominant Qy shift appears in the range 683-686 nm depending on species, and does not appear to have a systematic shift. Low-temperature absorption, circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of the chlorophyll Qy region are very similar in spinach and pea, but vary in cyanobacteria. We assigned CP43 and CP47 trap-chlorophyll absorption features in all species, as well as a P680 transition. Each absorption identified has an area of one chlorophyll a. The MCD deficit, introduced previously for spinach as an indicator of P680 activity, occurs in the same spectral region and has the same area in all species, pointing to a robustness of this as a signature for P680. MCD and CD characteristics point towards a significant variance in P680 structure between cyanobacteria, thermophilic cyanobacteria, and higher plants.",
keywords = "CD, Chlorophyll, Electrochromic shift, MCD, P680, Pea, Pheophytin, PS II, Spinach, Synechocystis PCC 6803, Thermosynechococcus vulcanus",
author = "{Peterson {\AA}rsk{\"o}ld}, Sindra and Smith, {Paul J.} and Jian-Ren Shen and Pace, {Ron J.} and Elmars Krausz",
year = "2005",
month = "6",
doi = "10.1007/s11120-005-2135-6",
language = "English",
volume = "84",
pages = "309--316",
journal = "Photosynthesis Research",
issn = "0166-8595",
publisher = "Springer Netherlands",
number = "1-3",

}

TY - JOUR

T1 - Key cofactors of Photosystem II cores from four organisms identified by 1.7-K absorption, CD and MCD

AU - Peterson Årsköld, Sindra

AU - Smith, Paul J.

AU - Shen, Jian-Ren

AU - Pace, Ron J.

AU - Krausz, Elmars

PY - 2005/6

Y1 - 2005/6

N2 - Active Photosystem II (PS II) cores were prepared from spinach, pea, Synechocystis PCC 6803, and Thermosynechococcus vulcanus, the latter of which has been structurally determined [Kamiya and Shen (2003) Proc Natl Acad Sci USA 100: 98-103]. Electrochromic shifts resulting from QA reduction by 1.7-K illumination were recorded, and the Qx and Qy absorption bands of the redox-active pheophytin a thus identified in the different organisms. The Qx transition is ∼3 nm (100 cm -1) to higher energy in cyanobacteria than in the plants. The predominant Qy shift appears in the range 683-686 nm depending on species, and does not appear to have a systematic shift. Low-temperature absorption, circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of the chlorophyll Qy region are very similar in spinach and pea, but vary in cyanobacteria. We assigned CP43 and CP47 trap-chlorophyll absorption features in all species, as well as a P680 transition. Each absorption identified has an area of one chlorophyll a. The MCD deficit, introduced previously for spinach as an indicator of P680 activity, occurs in the same spectral region and has the same area in all species, pointing to a robustness of this as a signature for P680. MCD and CD characteristics point towards a significant variance in P680 structure between cyanobacteria, thermophilic cyanobacteria, and higher plants.

AB - Active Photosystem II (PS II) cores were prepared from spinach, pea, Synechocystis PCC 6803, and Thermosynechococcus vulcanus, the latter of which has been structurally determined [Kamiya and Shen (2003) Proc Natl Acad Sci USA 100: 98-103]. Electrochromic shifts resulting from QA reduction by 1.7-K illumination were recorded, and the Qx and Qy absorption bands of the redox-active pheophytin a thus identified in the different organisms. The Qx transition is ∼3 nm (100 cm -1) to higher energy in cyanobacteria than in the plants. The predominant Qy shift appears in the range 683-686 nm depending on species, and does not appear to have a systematic shift. Low-temperature absorption, circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of the chlorophyll Qy region are very similar in spinach and pea, but vary in cyanobacteria. We assigned CP43 and CP47 trap-chlorophyll absorption features in all species, as well as a P680 transition. Each absorption identified has an area of one chlorophyll a. The MCD deficit, introduced previously for spinach as an indicator of P680 activity, occurs in the same spectral region and has the same area in all species, pointing to a robustness of this as a signature for P680. MCD and CD characteristics point towards a significant variance in P680 structure between cyanobacteria, thermophilic cyanobacteria, and higher plants.

KW - CD

KW - Chlorophyll

KW - Electrochromic shift

KW - MCD

KW - P680

KW - Pea

KW - Pheophytin

KW - PS II

KW - Spinach

KW - Synechocystis PCC 6803

KW - Thermosynechococcus vulcanus

UR - http://www.scopus.com/inward/record.url?scp=23444460413&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23444460413&partnerID=8YFLogxK

U2 - 10.1007/s11120-005-2135-6

DO - 10.1007/s11120-005-2135-6

M3 - Article

VL - 84

SP - 309

EP - 316

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

IS - 1-3

ER -