Karyotyping methods for fungi

Rahim Mehrabi, Masatoki Taga, Mostafa Aghaee, Pierre J.G.M. De Wit, Gert H.J. Kema

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Pulsed field gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes.

Original languageEnglish
Title of host publicationPlant Fungal Pathogens
Subtitle of host publicationMethods and Protocols
Pages591-602
Number of pages12
DOIs
Publication statusPublished - Jan 20 2012

Publication series

NameMethods in Molecular Biology
Volume835
ISSN (Print)1064-3745

Keywords

  • Chromosome polymorphisms
  • Electrophoretic karyotyping
  • Germ tube burst method
  • Pulsed field gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Mehrabi, R., Taga, M., Aghaee, M., De Wit, P. J. G. M., & Kema, G. H. J. (2012). Karyotyping methods for fungi. In Plant Fungal Pathogens: Methods and Protocols (pp. 591-602). (Methods in Molecular Biology; Vol. 835). https://doi.org/10.1007/978-1-61779-501-5_37