Juxta-epithelial hyalinization inhibits tumor growth and invasion in ameloblastoma

Gul San Ara Sathi, Masae Fujii, Silvia Susana Borkosky Ryo Tamamura, Naoki Katase, Toshiyuki Kawakami, Hitoshi Nagatsuka, Noriyuki Nagai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In histological examination of ameloblastoma, marked juxta-epithelial hyalinization of the connective tissue adjacent to the epithelium was observed in many cases. However, the physicochemical mechanism of these changes and its role in ameloblastoma is still not understood. To analyze the expression pattern of the different types of basement membrane related molecules, apoptosis-related factors and their possible role in juxta-epithelial hyalinization, six cases of ameloblastoma were examined. Immunohistochemical staining was done using cell surface type heparan sulfate (HS), 10E4, basement membrane type HS, JM403, Heparanase, Caspase-6, BCL-2 and CD34 antibodies. Hyalinized area in all sections, were strongly positive to 10E4, but negative to heparanase. There was no CD34 positive endothelial cells within the hyalinization area. Tumor cells adjacent to this area were positive to 10E4, heparanase and caspase-6. This result suggests that hyalinization have an effect on tumor growth and stop stromal-tumor cell interaction by the help of HSPGs, resulting in the inhibition of the function of heparanase and angiogenesis. Finally, tumor cells adjacent to the hyalinization undergoes program cell death.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalJournal of Hard Tissue Biology
Volume17
Issue number2
DOIs
Publication statusPublished - 2008

Fingerprint

Ameloblastoma
Tumors
Caspase 6
Heparitin Sulfate
Cells
Cell death
Growth
Basement Membrane
Neoplasms
Heparan Sulfate Proteoglycans
Endothelial cells
Stromal Cells
Antibodies
Cell Communication
Connective Tissue
Cell Death
Epithelium
Endothelial Cells
Tissue
Apoptosis

Keywords

  • Ameloblastoma
  • Angiogenesis
  • Apoptosis
  • Heparan sulfate proteoglycan (hspg)
  • Hyalinization

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

Sathi, G. S. A., Fujii, M., Ryo Tamamura, S. S. B., Katase, N., Kawakami, T., Nagatsuka, H., & Nagai, N. (2008). Juxta-epithelial hyalinization inhibits tumor growth and invasion in ameloblastoma. Journal of Hard Tissue Biology, 17(2), 63-68. https://doi.org/10.2485/jhtb.17.63

Juxta-epithelial hyalinization inhibits tumor growth and invasion in ameloblastoma. / Sathi, Gul San Ara; Fujii, Masae; Ryo Tamamura, Silvia Susana Borkosky; Katase, Naoki; Kawakami, Toshiyuki; Nagatsuka, Hitoshi; Nagai, Noriyuki.

In: Journal of Hard Tissue Biology, Vol. 17, No. 2, 2008, p. 63-68.

Research output: Contribution to journalArticle

Sathi, GSA, Fujii, M, Ryo Tamamura, SSB, Katase, N, Kawakami, T, Nagatsuka, H & Nagai, N 2008, 'Juxta-epithelial hyalinization inhibits tumor growth and invasion in ameloblastoma', Journal of Hard Tissue Biology, vol. 17, no. 2, pp. 63-68. https://doi.org/10.2485/jhtb.17.63
Sathi, Gul San Ara ; Fujii, Masae ; Ryo Tamamura, Silvia Susana Borkosky ; Katase, Naoki ; Kawakami, Toshiyuki ; Nagatsuka, Hitoshi ; Nagai, Noriyuki. / Juxta-epithelial hyalinization inhibits tumor growth and invasion in ameloblastoma. In: Journal of Hard Tissue Biology. 2008 ; Vol. 17, No. 2. pp. 63-68.
@article{3f5e01a671db48648f9301e3bfae3fec,
title = "Juxta-epithelial hyalinization inhibits tumor growth and invasion in ameloblastoma",
abstract = "In histological examination of ameloblastoma, marked juxta-epithelial hyalinization of the connective tissue adjacent to the epithelium was observed in many cases. However, the physicochemical mechanism of these changes and its role in ameloblastoma is still not understood. To analyze the expression pattern of the different types of basement membrane related molecules, apoptosis-related factors and their possible role in juxta-epithelial hyalinization, six cases of ameloblastoma were examined. Immunohistochemical staining was done using cell surface type heparan sulfate (HS), 10E4, basement membrane type HS, JM403, Heparanase, Caspase-6, BCL-2 and CD34 antibodies. Hyalinized area in all sections, were strongly positive to 10E4, but negative to heparanase. There was no CD34 positive endothelial cells within the hyalinization area. Tumor cells adjacent to this area were positive to 10E4, heparanase and caspase-6. This result suggests that hyalinization have an effect on tumor growth and stop stromal-tumor cell interaction by the help of HSPGs, resulting in the inhibition of the function of heparanase and angiogenesis. Finally, tumor cells adjacent to the hyalinization undergoes program cell death.",
keywords = "Ameloblastoma, Angiogenesis, Apoptosis, Heparan sulfate proteoglycan (hspg), Hyalinization",
author = "Sathi, {Gul San Ara} and Masae Fujii and {Ryo Tamamura}, {Silvia Susana Borkosky} and Naoki Katase and Toshiyuki Kawakami and Hitoshi Nagatsuka and Noriyuki Nagai",
year = "2008",
doi = "10.2485/jhtb.17.63",
language = "English",
volume = "17",
pages = "63--68",
journal = "Journal of Hard Tissue Biology",
issn = "1341-7649",
publisher = "Society of Hard Tissue Regenerative Biology",
number = "2",

}

TY - JOUR

T1 - Juxta-epithelial hyalinization inhibits tumor growth and invasion in ameloblastoma

AU - Sathi, Gul San Ara

AU - Fujii, Masae

AU - Ryo Tamamura, Silvia Susana Borkosky

AU - Katase, Naoki

AU - Kawakami, Toshiyuki

AU - Nagatsuka, Hitoshi

AU - Nagai, Noriyuki

PY - 2008

Y1 - 2008

N2 - In histological examination of ameloblastoma, marked juxta-epithelial hyalinization of the connective tissue adjacent to the epithelium was observed in many cases. However, the physicochemical mechanism of these changes and its role in ameloblastoma is still not understood. To analyze the expression pattern of the different types of basement membrane related molecules, apoptosis-related factors and their possible role in juxta-epithelial hyalinization, six cases of ameloblastoma were examined. Immunohistochemical staining was done using cell surface type heparan sulfate (HS), 10E4, basement membrane type HS, JM403, Heparanase, Caspase-6, BCL-2 and CD34 antibodies. Hyalinized area in all sections, were strongly positive to 10E4, but negative to heparanase. There was no CD34 positive endothelial cells within the hyalinization area. Tumor cells adjacent to this area were positive to 10E4, heparanase and caspase-6. This result suggests that hyalinization have an effect on tumor growth and stop stromal-tumor cell interaction by the help of HSPGs, resulting in the inhibition of the function of heparanase and angiogenesis. Finally, tumor cells adjacent to the hyalinization undergoes program cell death.

AB - In histological examination of ameloblastoma, marked juxta-epithelial hyalinization of the connective tissue adjacent to the epithelium was observed in many cases. However, the physicochemical mechanism of these changes and its role in ameloblastoma is still not understood. To analyze the expression pattern of the different types of basement membrane related molecules, apoptosis-related factors and their possible role in juxta-epithelial hyalinization, six cases of ameloblastoma were examined. Immunohistochemical staining was done using cell surface type heparan sulfate (HS), 10E4, basement membrane type HS, JM403, Heparanase, Caspase-6, BCL-2 and CD34 antibodies. Hyalinized area in all sections, were strongly positive to 10E4, but negative to heparanase. There was no CD34 positive endothelial cells within the hyalinization area. Tumor cells adjacent to this area were positive to 10E4, heparanase and caspase-6. This result suggests that hyalinization have an effect on tumor growth and stop stromal-tumor cell interaction by the help of HSPGs, resulting in the inhibition of the function of heparanase and angiogenesis. Finally, tumor cells adjacent to the hyalinization undergoes program cell death.

KW - Ameloblastoma

KW - Angiogenesis

KW - Apoptosis

KW - Heparan sulfate proteoglycan (hspg)

KW - Hyalinization

UR - http://www.scopus.com/inward/record.url?scp=70349115014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349115014&partnerID=8YFLogxK

U2 - 10.2485/jhtb.17.63

DO - 10.2485/jhtb.17.63

M3 - Article

AN - SCOPUS:70349115014

VL - 17

SP - 63

EP - 68

JO - Journal of Hard Tissue Biology

JF - Journal of Hard Tissue Biology

SN - 1341-7649

IS - 2

ER -