Joint development in Xenopus laevis and induction of segmentations in regenerating froglet limb (spike)

Akira Sato, Makoto Suzuki, Takanori Amano, Koji Tamura, Hiroyuki Ide

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In Xenopus laevis, amputation of the adult limb results in the formation of a simple (hypomorphic) spike-like structure without joints, although tadpole limb bud regenerates complete limb pattern. The expression of some joint marker genes was examined in limb development and regeneration. Bmp-4 and gdf-5 were expressed and sox-9 expression was decreased in the joint region. Although developing cartilages were well-organized and had bmp-4 expressing perichondrocytes, the spike cartilage did not have such a structure, but only showed sparse bmp-4 expression. Application of BMP4-soaked beads to the spike led to the induction of a joint-like structure. These results suggest that the lack of joints in the spike is due to the deficiency of the accumulation of the cells that express bmp-4. Improvement of regeneration in the Xenopus adult limb that we report here for the first time will give us important insights into epimorphic regeneration.

Original languageEnglish
Pages (from-to)1444-1453
Number of pages10
JournalDevelopmental Dynamics
Volume233
Issue number4
DOIs
Publication statusPublished - Aug 2005
Externally publishedYes

Fingerprint

Xenopus laevis
Extremities
Joints
Regeneration
Cartilage
Limb Buds
Xenopus
Amputation
Larva
Genes

Keywords

  • Bmp-4
  • Gdf-
  • Joint
  • Limb
  • Regeneration
  • Sox-9
  • Spike
  • Xenopus laevis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Joint development in Xenopus laevis and induction of segmentations in regenerating froglet limb (spike). / Sato, Akira; Suzuki, Makoto; Amano, Takanori; Tamura, Koji; Ide, Hiroyuki.

In: Developmental Dynamics, Vol. 233, No. 4, 08.2005, p. 1444-1453.

Research output: Contribution to journalArticle

Sato, Akira ; Suzuki, Makoto ; Amano, Takanori ; Tamura, Koji ; Ide, Hiroyuki. / Joint development in Xenopus laevis and induction of segmentations in regenerating froglet limb (spike). In: Developmental Dynamics. 2005 ; Vol. 233, No. 4. pp. 1444-1453.
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