Jiadifenolide induces the expression of cellular communication network factor (CCN) genes, and CCN2 exhibits neurotrophic activity in neuronal precursor cells derived from human induced pluripotent stem cells

Masaki Shoji; Masako Ueda; Megumi Nishioka; Hiroki Minato; Masahide Seki; Kenichi Harada; Miwa Kubo; Yoshiyasu Fukuyama; Yutaka Suzuki; Eriko Aoyama; Masaharu Takigawa; Takashi Kuzuhara

doi:10.1016/j.bbrc.2019.09.003

Jiadifenolide induces the expression of cellular communication network factor (CCN) genes, and CCN2 exhibits neurotrophic activity in neuronal precursor cells derived from human induced pluripotent stem cells

Masaki Shoji, Masako Ueda, Megumi Nishioka, Hiroki Minato, Masahide Seki, Kenichi Harada, Miwa Kubo, Yoshiyasu Fukuyama, Yutaka Suzuki, Eriko Aoyama, Masaharu Takigawa, Takashi Kuzuhara

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Jiadifenolide has been reported to have neurotrophin-like activity in primary rat cortical neurons, and also possesses neurotrophic effects in neuronal precursor cells derived from human induced pluripotent stem cells (hiPSCs), as we have previously reported. However, the molecular mechanisms by which jiadifenolide exerts its neurotrophic effects in rat and human neurons are unknown. Thus, we aimed to investigate the molecular mechanisms and pathways by which jiadifenolide promotes neurotrophic effects. Here, we found that jiadifenolide activated cellular communication network factor (CCN) signaling pathways by up-regulating mRNA level expression of CCN genes in human neuronal cells. We also found that CCN2 (also known as connective tissue growth factor, CTGF) protein promotes neurotrophic effects through activation of the p44/42 mitogen-activated protein kinase signaling pathway. This is the first discovery which links neurotrophic activity with CCN signaling.

Original language	English
Pages (from-to)	309-315
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	519
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2019.09.003
Publication status	Published - Nov 5 2019

Keywords

CCN signaling pathway
CCN2
Jiadifenolide
Neurotrophin
iPS

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Shoji, M., Ueda, M., Nishioka, M., Minato, H., Seki, M., Harada, K., Kubo, M., Fukuyama, Y., Suzuki, Y., Aoyama, E., Takigawa, M., & Kuzuhara, T. (2019). Jiadifenolide induces the expression of cellular communication network factor (CCN) genes, and CCN2 exhibits neurotrophic activity in neuronal precursor cells derived from human induced pluripotent stem cells. Biochemical and Biophysical Research Communications, 519(2), 309-315. https://doi.org/10.1016/j.bbrc.2019.09.003

Jiadifenolide induces the expression of cellular communication network factor (CCN) genes, and CCN2 exhibits neurotrophic activity in neuronal precursor cells derived from human induced pluripotent stem cells. / Shoji, Masaki; Ueda, Masako; Nishioka, Megumi et al.

In: Biochemical and Biophysical Research Communications, Vol. 519, No. 2, 05.11.2019, p. 309-315.

Research output: Contribution to journal › Article › peer-review

Shoji, M, Ueda, M, Nishioka, M, Minato, H, Seki, M, Harada, K, Kubo, M, Fukuyama, Y, Suzuki, Y, Aoyama, E , Takigawa, M & Kuzuhara, T 2019, 'Jiadifenolide induces the expression of cellular communication network factor (CCN) genes, and CCN2 exhibits neurotrophic activity in neuronal precursor cells derived from human induced pluripotent stem cells', Biochemical and Biophysical Research Communications, vol. 519, no. 2, pp. 309-315. https://doi.org/10.1016/j.bbrc.2019.09.003

Shoji M, Ueda M, Nishioka M, Minato H, Seki M, Harada K et al. Jiadifenolide induces the expression of cellular communication network factor (CCN) genes, and CCN2 exhibits neurotrophic activity in neuronal precursor cells derived from human induced pluripotent stem cells. Biochemical and Biophysical Research Communications. 2019 Nov 5;519(2):309-315. doi: 10.1016/j.bbrc.2019.09.003

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title = "Jiadifenolide induces the expression of cellular communication network factor (CCN) genes, and CCN2 exhibits neurotrophic activity in neuronal precursor cells derived from human induced pluripotent stem cells",

abstract = "Jiadifenolide has been reported to have neurotrophin-like activity in primary rat cortical neurons, and also possesses neurotrophic effects in neuronal precursor cells derived from human induced pluripotent stem cells (hiPSCs), as we have previously reported. However, the molecular mechanisms by which jiadifenolide exerts its neurotrophic effects in rat and human neurons are unknown. Thus, we aimed to investigate the molecular mechanisms and pathways by which jiadifenolide promotes neurotrophic effects. Here, we found that jiadifenolide activated cellular communication network factor (CCN) signaling pathways by up-regulating mRNA level expression of CCN genes in human neuronal cells. We also found that CCN2 (also known as connective tissue growth factor, CTGF) protein promotes neurotrophic effects through activation of the p44/42 mitogen-activated protein kinase signaling pathway. This is the first discovery which links neurotrophic activity with CCN signaling.",

keywords = "CCN signaling pathway, CCN2, Jiadifenolide, Neurotrophin, iPS",

author = "Masaki Shoji and Masako Ueda and Megumi Nishioka and Hiroki Minato and Masahide Seki and Kenichi Harada and Miwa Kubo and Yoshiyasu Fukuyama and Yutaka Suzuki and Eriko Aoyama and Masaharu Takigawa and Takashi Kuzuhara",

note = "Funding Information: This work was supported by the Japan Society for the Promotion of Science (JSPS), Grants-in-Aid for Scientific Research (C) 18K06645 (to T.K.) and (B) 19H03817 (to M.T.), Grants-in-Aid for Young Scientists (B) 26860083 (to M.S.), the Tokushima Bunri University Grant for Educational Reform, and the Collaborative Research Grants TBU2015-2-2 (to M.S.). Publisher Copyright: {\textcopyright} 2019 The Authors",

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AU - Shoji, Masaki

AU - Ueda, Masako

AU - Nishioka, Megumi

AU - Minato, Hiroki

AU - Seki, Masahide

AU - Harada, Kenichi

AU - Kubo, Miwa

AU - Fukuyama, Yoshiyasu

AU - Suzuki, Yutaka

AU - Aoyama, Eriko

AU - Takigawa, Masaharu

AU - Kuzuhara, Takashi

N1 - Funding Information: This work was supported by the Japan Society for the Promotion of Science (JSPS), Grants-in-Aid for Scientific Research (C) 18K06645 (to T.K.) and (B) 19H03817 (to M.T.), Grants-in-Aid for Young Scientists (B) 26860083 (to M.S.), the Tokushima Bunri University Grant for Educational Reform, and the Collaborative Research Grants TBU2015-2-2 (to M.S.). Publisher Copyright: © 2019 The Authors

PY - 2019/11/5

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N2 - Jiadifenolide has been reported to have neurotrophin-like activity in primary rat cortical neurons, and also possesses neurotrophic effects in neuronal precursor cells derived from human induced pluripotent stem cells (hiPSCs), as we have previously reported. However, the molecular mechanisms by which jiadifenolide exerts its neurotrophic effects in rat and human neurons are unknown. Thus, we aimed to investigate the molecular mechanisms and pathways by which jiadifenolide promotes neurotrophic effects. Here, we found that jiadifenolide activated cellular communication network factor (CCN) signaling pathways by up-regulating mRNA level expression of CCN genes in human neuronal cells. We also found that CCN2 (also known as connective tissue growth factor, CTGF) protein promotes neurotrophic effects through activation of the p44/42 mitogen-activated protein kinase signaling pathway. This is the first discovery which links neurotrophic activity with CCN signaling.

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Jiadifenolide induces the expression of cellular communication network factor (CCN) genes, and CCN2 exhibits neurotrophic activity in neuronal precursor cells derived from human induced pluripotent stem cells

Abstract

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