Jak1 plays an essential role for receptor phosphorylation and stat activation in response to granulocyte colony-stimulating factor

Kazuya Shimoda, Jian Feng, Hiroshi Murakami, Shigekazu Nagata, Diane Watling, Neil C. Rogers, George R. Stark, Ian M. Kerr, James N. Ihle

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

The proliferation and differentiation of neutrophils is regulated by granulocyte-specific colony-stimulating factor (G-CSF). G-CSF uses a receptor of the cytokine receptor superfamily and, in common with all members of the family, induces the tyrosine phosphorylation and activation of members of the Janus protein tyrosine kinase (Jak) family. In both myeloid cells and a human fibrosarcoma cell line expressing the G-CSF receptor, G-CSF induces the tyrosine phosphorylation and activation of Jak1, Jak2, and Tyk2. In addition, G-CSF induces the tyrosine phosphorylation of the receptor and members of the signal transducers and activators of transcription (Stat) family, including Stat3, as well as Stat1 and Stat5, depending on the cells involved. Using mutant cell lines lacking various Jaks, we show here that Jak1 is critical for G-CSF-mediated Stat activation, whereas Jak2 or Tyk2 are either not required or play redundant or ancillary roles. In the absence of Jak1, G-CSF induces activation of Jak2 and Tyk2, but fails to induce receptor tyrosine phosphorylation and induces dramatically reduced levels of Stat activation. A kinase-inactive Jak2, when overexpressed in cells lacking endogenous Jak2, can suppress Jak1 activation, receptor phosphorylation, and Stat activation, suggesting competition in the receptor complex either for Jak1 binding or substrates. Because the requirement for Jak1 is very similar to that previously shown for interleukin-6 signaling, the data support the concept that the G-CSF receptor and gp130 are both structurally and functionally similar.

Original languageEnglish
Pages (from-to)597-604
Number of pages8
JournalBlood
Volume90
Issue number2
Publication statusPublished - Jul 15 1997
Externally publishedYes

Fingerprint

Phosphorylation
Colony-Stimulating Factors
Granulocyte Colony-Stimulating Factor
Chemical activation
Transducers
Transcription
Granulocyte Colony-Stimulating Factor Receptors
Transcriptional Activation
Tyrosine
Janus Kinases
Colony-Stimulating Factor Receptors
Cell Line
Cells
Cytokine Receptors
Fibrosarcoma
Myeloid Cells
Protein-Tyrosine Kinases
Interleukin-6
Neutrophils
Phosphotransferases

ASJC Scopus subject areas

  • Hematology

Cite this

Shimoda, K., Feng, J., Murakami, H., Nagata, S., Watling, D., Rogers, N. C., ... Ihle, J. N. (1997). Jak1 plays an essential role for receptor phosphorylation and stat activation in response to granulocyte colony-stimulating factor. Blood, 90(2), 597-604.

Jak1 plays an essential role for receptor phosphorylation and stat activation in response to granulocyte colony-stimulating factor. / Shimoda, Kazuya; Feng, Jian; Murakami, Hiroshi; Nagata, Shigekazu; Watling, Diane; Rogers, Neil C.; Stark, George R.; Kerr, Ian M.; Ihle, James N.

In: Blood, Vol. 90, No. 2, 15.07.1997, p. 597-604.

Research output: Contribution to journalArticle

Shimoda, K, Feng, J, Murakami, H, Nagata, S, Watling, D, Rogers, NC, Stark, GR, Kerr, IM & Ihle, JN 1997, 'Jak1 plays an essential role for receptor phosphorylation and stat activation in response to granulocyte colony-stimulating factor', Blood, vol. 90, no. 2, pp. 597-604.
Shimoda, Kazuya ; Feng, Jian ; Murakami, Hiroshi ; Nagata, Shigekazu ; Watling, Diane ; Rogers, Neil C. ; Stark, George R. ; Kerr, Ian M. ; Ihle, James N. / Jak1 plays an essential role for receptor phosphorylation and stat activation in response to granulocyte colony-stimulating factor. In: Blood. 1997 ; Vol. 90, No. 2. pp. 597-604.
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