Isolation, purification, and characterization of a collagen-associated serpin, caspin, produced by murine colon adenocarcinoma cells

Ken Ichi Kozaki, Osamu Miyaishi, Osamu Koiwai, Yoshihiro Yasui, Akiko Kashiwai, Yohko Nishikawa, Satoru Shimizu, Shinsuke Saga

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Abstract

A 45-kDa serpin secreted by a murine colon adenocarcinoma cell line, colon26, was isolated, purified, and characterized. It was found to bind specifically to type I collagen with high affinity and to type III collagen with lower affinity. Immunohistochemical studies of murine embryonic tissues showed a specific distribution of this collagen-associated serpin, named caspin, in relation to the formation of bone, cartilage, teeth, and basement membrane. The expression of caspin in high and low lung metastatic subclones of colon26 cell lines was inversely correlated with their metastatic capacity: low lung metastatic cells secreted higher amounts of caspin than their high lung metastatic counterparts. Caspin also demonstrated high homology with human pigment epithelium-derived factor/early population doubling level cDNA-1, which reportedly induces neuronal differentiation of human retinoblastoma cells and is expressed in association with G0 growth arrest. These findings suggest that caspin/pigment epithelium-derived factor/early population doubling level cDNA-1 is a novel factor that might play a crucial role in embryo-genesis and tumor metastasis through binding to the extracellular matrix.

Original languageEnglish
Pages (from-to)15125-15130
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number24
DOIs
Publication statusPublished - Jun 12 1998
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Kozaki, K. I., Miyaishi, O., Koiwai, O., Yasui, Y., Kashiwai, A., Nishikawa, Y., Shimizu, S., & Saga, S. (1998). Isolation, purification, and characterization of a collagen-associated serpin, caspin, produced by murine colon adenocarcinoma cells. Journal of Biological Chemistry, 273(24), 15125-15130. https://doi.org/10.1074/jbc.273.24.15125