Isolation of virus-producing transformants from human gastric cancer cell line, HGC-27, infected with human T-cell leukemia virus type I

Tadaatsu Akagi, Tadashi Yoshino, Makoto Motoi, Hiroshi Takata, Shoki Yano, Isao Miyoshi, Takashi Oka, Yuji Ohtsuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A human anaplastic gastric cancer cell line, HGC-27, showed marked degeneration with formation of multinucleated syncytia and cell detachment of nearly all cells which began 24 hr after and reached a maximum 2 to 3 days after co-cultivation with X-irradiated MT-2 cells, HTLV-I producing human cord leukocytes. Less severe degeneration without formation of syncytia was also observed in the cultures inoculated with cell-free MT-2 culture media. Morphologically altered cells began to proliferate and formed piled up colonies in some of the cultures co-cultivated with X-irradiated MT-2 cells after a long culture period. The two clones designated HGC MT2 (Cl-1) and HGC MT2 (Cl-2) were separated by cell cloning. HGC MT2 (Cl-1) and HGC MT2 (Cl-2) cells were positive for HTLV-I gag proteins (p19 and p24) and pX gene products, p40x, as demonstrated by immunohistochemistry and immunoblotting analysis, contained HTLV-I provirus DNA, and consistently produced type C virus particles.

Original languageEnglish
Pages (from-to)836-842
Number of pages7
JournalJapanese Journal of Cancer Research
Volume79
Issue number7
Publication statusPublished - Jul 1988

Fingerprint

Human T-lymphotropic virus 1
Stomach Neoplasms
Viruses
Cell Line
Giant Cells
pX Genes
Proviruses
Immunoblotting
Virion
Culture Media
Organism Cloning
Leukocytes
Clone Cells
Immunohistochemistry
DNA

Keywords

  • Cell line
  • Gastric cancer
  • Human T-cell leukemia virus type I
  • Transformation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Isolation of virus-producing transformants from human gastric cancer cell line, HGC-27, infected with human T-cell leukemia virus type I. / Akagi, Tadaatsu; Yoshino, Tadashi; Motoi, Makoto; Takata, Hiroshi; Yano, Shoki; Miyoshi, Isao; Oka, Takashi; Ohtsuki, Yuji.

In: Japanese Journal of Cancer Research, Vol. 79, No. 7, 07.1988, p. 836-842.

Research output: Contribution to journalArticle

Akagi, Tadaatsu ; Yoshino, Tadashi ; Motoi, Makoto ; Takata, Hiroshi ; Yano, Shoki ; Miyoshi, Isao ; Oka, Takashi ; Ohtsuki, Yuji. / Isolation of virus-producing transformants from human gastric cancer cell line, HGC-27, infected with human T-cell leukemia virus type I. In: Japanese Journal of Cancer Research. 1988 ; Vol. 79, No. 7. pp. 836-842.
@article{eb73d3e613d54ca5870959e4a3d77800,
title = "Isolation of virus-producing transformants from human gastric cancer cell line, HGC-27, infected with human T-cell leukemia virus type I",
abstract = "A human anaplastic gastric cancer cell line, HGC-27, showed marked degeneration with formation of multinucleated syncytia and cell detachment of nearly all cells which began 24 hr after and reached a maximum 2 to 3 days after co-cultivation with X-irradiated MT-2 cells, HTLV-I producing human cord leukocytes. Less severe degeneration without formation of syncytia was also observed in the cultures inoculated with cell-free MT-2 culture media. Morphologically altered cells began to proliferate and formed piled up colonies in some of the cultures co-cultivated with X-irradiated MT-2 cells after a long culture period. The two clones designated HGC MT2 (Cl-1) and HGC MT2 (Cl-2) were separated by cell cloning. HGC MT2 (Cl-1) and HGC MT2 (Cl-2) cells were positive for HTLV-I gag proteins (p19 and p24) and pX gene products, p40x, as demonstrated by immunohistochemistry and immunoblotting analysis, contained HTLV-I provirus DNA, and consistently produced type C virus particles.",
keywords = "Cell line, Gastric cancer, Human T-cell leukemia virus type I, Transformation",
author = "Tadaatsu Akagi and Tadashi Yoshino and Makoto Motoi and Hiroshi Takata and Shoki Yano and Isao Miyoshi and Takashi Oka and Yuji Ohtsuki",
year = "1988",
month = "7",
language = "English",
volume = "79",
pages = "836--842",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Isolation of virus-producing transformants from human gastric cancer cell line, HGC-27, infected with human T-cell leukemia virus type I

AU - Akagi, Tadaatsu

AU - Yoshino, Tadashi

AU - Motoi, Makoto

AU - Takata, Hiroshi

AU - Yano, Shoki

AU - Miyoshi, Isao

AU - Oka, Takashi

AU - Ohtsuki, Yuji

PY - 1988/7

Y1 - 1988/7

N2 - A human anaplastic gastric cancer cell line, HGC-27, showed marked degeneration with formation of multinucleated syncytia and cell detachment of nearly all cells which began 24 hr after and reached a maximum 2 to 3 days after co-cultivation with X-irradiated MT-2 cells, HTLV-I producing human cord leukocytes. Less severe degeneration without formation of syncytia was also observed in the cultures inoculated with cell-free MT-2 culture media. Morphologically altered cells began to proliferate and formed piled up colonies in some of the cultures co-cultivated with X-irradiated MT-2 cells after a long culture period. The two clones designated HGC MT2 (Cl-1) and HGC MT2 (Cl-2) were separated by cell cloning. HGC MT2 (Cl-1) and HGC MT2 (Cl-2) cells were positive for HTLV-I gag proteins (p19 and p24) and pX gene products, p40x, as demonstrated by immunohistochemistry and immunoblotting analysis, contained HTLV-I provirus DNA, and consistently produced type C virus particles.

AB - A human anaplastic gastric cancer cell line, HGC-27, showed marked degeneration with formation of multinucleated syncytia and cell detachment of nearly all cells which began 24 hr after and reached a maximum 2 to 3 days after co-cultivation with X-irradiated MT-2 cells, HTLV-I producing human cord leukocytes. Less severe degeneration without formation of syncytia was also observed in the cultures inoculated with cell-free MT-2 culture media. Morphologically altered cells began to proliferate and formed piled up colonies in some of the cultures co-cultivated with X-irradiated MT-2 cells after a long culture period. The two clones designated HGC MT2 (Cl-1) and HGC MT2 (Cl-2) were separated by cell cloning. HGC MT2 (Cl-1) and HGC MT2 (Cl-2) cells were positive for HTLV-I gag proteins (p19 and p24) and pX gene products, p40x, as demonstrated by immunohistochemistry and immunoblotting analysis, contained HTLV-I provirus DNA, and consistently produced type C virus particles.

KW - Cell line

KW - Gastric cancer

KW - Human T-cell leukemia virus type I

KW - Transformation

UR - http://www.scopus.com/inward/record.url?scp=0023690514&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023690514&partnerID=8YFLogxK

M3 - Article

VL - 79

SP - 836

EP - 842

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 7

ER -