Isolation of viable but nonculturable Vibrio cholerae O1 from environmental water samples in Kolkata, India, in a culturable state

Mitsutoshi Senoh, Jayeeta Ghosh-Banerjee, Tamaki Mizuno, Sumio Shinoda, Shin-ichi Miyoshi, Takashi Hamabata, G. Balakrish Nair, Yoshifumi Takeda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Previously, we reported that viable but nonculturable (VBNC) Vibrio cholerae was converted into a culturable state by coculture with several eukaryotic cell lines including HT-29 cells. In this study, we found that a factor converting VBNC V. cholerae into a culturable state (FCVC) existed in cell extracts of eukaryotic cells. FCVC was nondialyzable, proteinase K-sensitive, and stable to heating at

Original languageEnglish
Pages (from-to)239-246
Number of pages8
JournalMicrobiologyOpen
Volume3
Issue number2
DOIs
Publication statusPublished - 2014

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Vibrio cholerae O1
Vibrio cholerae
Eukaryotic Cells
India
Endopeptidase K
HT29 Cells
Water
Coculture Techniques
Cell Extracts
Heating
Cell Line

Keywords

  • Factor converting VBNC into culturable
  • VBNC vibrio cholerae

ASJC Scopus subject areas

  • Microbiology
  • Medicine(all)

Cite this

Isolation of viable but nonculturable Vibrio cholerae O1 from environmental water samples in Kolkata, India, in a culturable state. / Senoh, Mitsutoshi; Ghosh-Banerjee, Jayeeta; Mizuno, Tamaki; Shinoda, Sumio; Miyoshi, Shin-ichi; Hamabata, Takashi; Nair, G. Balakrish; Takeda, Yoshifumi.

In: MicrobiologyOpen, Vol. 3, No. 2, 2014, p. 239-246.

Research output: Contribution to journalArticle

Senoh, Mitsutoshi ; Ghosh-Banerjee, Jayeeta ; Mizuno, Tamaki ; Shinoda, Sumio ; Miyoshi, Shin-ichi ; Hamabata, Takashi ; Nair, G. Balakrish ; Takeda, Yoshifumi. / Isolation of viable but nonculturable Vibrio cholerae O1 from environmental water samples in Kolkata, India, in a culturable state. In: MicrobiologyOpen. 2014 ; Vol. 3, No. 2. pp. 239-246.
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