Isolation of three kinds of human endogenous retrovirus-like sequences using tRNAPro as a probe

Fumio Harada, Naoko Tsukada, Nobuyuki Kato

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Three kinds of human endogenous retrovirus-like sequences (HuERS-Pl, 2 and 3) were isolated from a HeLa cell genomic library using the 3′-half fragment of proline tRNA as a hybridization probe. These elements contained putative primer binding sites complementary to the 3′-terminus of proline tRNA and long terminal repeats (LTRs) characteristic of retrovirus provirus. The LTR sequence of HuERS-Pl consisted of about 690 nucleotides and contained a CAT box, a TATA box and a polyadenylation signal. A complete unit of an Alu family sequence was inserted into the 5′-LTR of one of the clones. HuERS-P2 also contained a TATA box and a polyadenylation signal in its LTR (about 840 nucleotides long), but the LTR sequence of this element was quite different from that of HuERS-Pl. Although clone HuERS-P3 contained only the 5′-LTR region, this LTR sequence contained a CAT box, a TATA box and a polyadenylation signal and was quite similar to the LTR sequence of the recently isolated human retrovirus-related sequence HuRRS-P (Kröger, B. and Horak, I. (1987) J. Virol., 61, 2071-2075). Human and simian DNAs contain 10 to 40 copies of these elements, but mouse DNA does not contain these elements.

Original languageEnglish
Pages (from-to)9153-9162
Number of pages10
JournalNucleic acids research.
Volume15
Issue number22
DOIs
Publication statusPublished - Nov 25 1987
Externally publishedYes

Fingerprint

RNA, Transfer, Pro
Endogenous Retroviruses
Terminal Repeat Sequences
Nucleotides
Isolation
DNA
Probe
Binding sites
TATA Box
Polyadenylation
Clone
Retroviridae
Transfer RNA
Proline
Human
Clone Cells
Genomics
Mouse
Alu Elements
Fragment

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Isolation of three kinds of human endogenous retrovirus-like sequences using tRNAPro as a probe. / Harada, Fumio; Tsukada, Naoko; Kato, Nobuyuki.

In: Nucleic acids research., Vol. 15, No. 22, 25.11.1987, p. 9153-9162.

Research output: Contribution to journalArticle

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