Isolation of tetracycline-resistant clinical Helicobacter pylori without mutations in 16S rRNA gene in Bangladesh

Rasel Khan; Shamsun Nahar; Asish K. Mukhopadhyay; Douglas E. Berg; Mian Mashhud Ahmad; Keinosuke Okamoto; G. Balakrish Nair; Motiur Rahman

doi:10.1111/j.1348-0421.2008.00062.x

Isolation of tetracycline-resistant clinical Helicobacter pylori without mutations in 16S rRNA gene in Bangladesh

Rasel Khan, Shamsun Nahar, Asish K. Mukhopadhyay, Douglas E. Berg, Mian Mashhud Ahmad, Keinosuke Okamoto, G. Balakrish Nair, Motiur Rahman

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The occurrence of 16S rRNA gene mutations associated with resistance to tetracycline in H. pylori isolated in Bangladesh was investigated. Tetracycline susceptibility was determined by the agar dilution method. The 16S rRNA genes of these isolates were sequenced and analyzed. A tetracycline accumulation assay was performed. DNA sequence and transformation tests of nine tetracycline-resistant (MIC = 2 μg/ml) Bangladeshi H. pylori clinical isolates showed that in no case was the resistance due to mutations in the 16S rRNA gene, the only known cause of tetracycline resistance in this pathogen. Tetracycline accumulation assays implicated altered uptake or efflux.

Original language	English
Pages (from-to)	508-511
Number of pages	4
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	52
Issue number	10
DOIs	https://doi.org/10.1111/j.1348-0421.2008.00062.x
Publication status	Published - Oct 2008
Externally published	Yes

Keywords

16S rRNA gene mutation
Helicobacter pylori
Resistance
Tetracycline

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.2008.00062.x

Cite this

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title = "Isolation of tetracycline-resistant clinical Helicobacter pylori without mutations in 16S rRNA gene in Bangladesh",

abstract = "The occurrence of 16S rRNA gene mutations associated with resistance to tetracycline in H. pylori isolated in Bangladesh was investigated. Tetracycline susceptibility was determined by the agar dilution method. The 16S rRNA genes of these isolates were sequenced and analyzed. A tetracycline accumulation assay was performed. DNA sequence and transformation tests of nine tetracycline-resistant (MIC = 2 μg/ml) Bangladeshi H. pylori clinical isolates showed that in no case was the resistance due to mutations in the 16S rRNA gene, the only known cause of tetracycline resistance in this pathogen. Tetracycline accumulation assays implicated altered uptake or efflux.",

keywords = "16S rRNA gene mutation, Helicobacter pylori, Resistance, Tetracycline",

author = "Rasel Khan and Shamsun Nahar and Mukhopadhyay, {Asish K.} and Berg, {Douglas E.} and Ahmad, {Mian Mashhud} and Keinosuke Okamoto and {Balakrish Nair}, G. and Motiur Rahman",

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T1 - Isolation of tetracycline-resistant clinical Helicobacter pylori without mutations in 16S rRNA gene in Bangladesh

AU - Khan, Rasel

AU - Nahar, Shamsun

AU - Mukhopadhyay, Asish K.

AU - Berg, Douglas E.

AU - Ahmad, Mian Mashhud

AU - Okamoto, Keinosuke

AU - Balakrish Nair, G.

AU - Rahman, Motiur

PY - 2008/10

Y1 - 2008/10

N2 - The occurrence of 16S rRNA gene mutations associated with resistance to tetracycline in H. pylori isolated in Bangladesh was investigated. Tetracycline susceptibility was determined by the agar dilution method. The 16S rRNA genes of these isolates were sequenced and analyzed. A tetracycline accumulation assay was performed. DNA sequence and transformation tests of nine tetracycline-resistant (MIC = 2 μg/ml) Bangladeshi H. pylori clinical isolates showed that in no case was the resistance due to mutations in the 16S rRNA gene, the only known cause of tetracycline resistance in this pathogen. Tetracycline accumulation assays implicated altered uptake or efflux.

AB - The occurrence of 16S rRNA gene mutations associated with resistance to tetracycline in H. pylori isolated in Bangladesh was investigated. Tetracycline susceptibility was determined by the agar dilution method. The 16S rRNA genes of these isolates were sequenced and analyzed. A tetracycline accumulation assay was performed. DNA sequence and transformation tests of nine tetracycline-resistant (MIC = 2 μg/ml) Bangladeshi H. pylori clinical isolates showed that in no case was the resistance due to mutations in the 16S rRNA gene, the only known cause of tetracycline resistance in this pathogen. Tetracycline accumulation assays implicated altered uptake or efflux.

KW - 16S rRNA gene mutation

KW - Helicobacter pylori

KW - Resistance

KW - Tetracycline

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Isolation of tetracycline-resistant clinical Helicobacter pylori without mutations in 16S rRNA gene in Bangladesh

Abstract

Keywords

ASJC Scopus subject areas

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