Isolation of small RNAs using biotinylated PNAs

Takashi Ohtsuki, Takeshi Fujimoto, Maya Kamimukai, Chisato Kumano, Mizuki Kitamatsu, Masahiko Sisido

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, an RNA isolation method was developed using a biotinylated peptide nucleic acid (PNA) that is complementary to the target RNA. Using the biotinylated PNA method, we successfully isolated several RNAs from Escherichia coli and from human total RNA in pure form. Damage to the RNA appears to be negligible by this method because the method is rapid and does not require a high temperature treatment to facilitate RNA-PNA binding.

Original languageEnglish
Pages (from-to)415-418
Number of pages4
JournalJournal of biochemistry
Volume144
Issue number4
DOIs
Publication statusPublished - Oct 2008

Keywords

  • Non-coding RNA
  • Peptide nucleic acid
  • RNA isolation
  • Ribosomal RNA
  • Transfer RNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Ohtsuki, T., Fujimoto, T., Kamimukai, M., Kumano, C., Kitamatsu, M., & Sisido, M. (2008). Isolation of small RNAs using biotinylated PNAs. Journal of biochemistry, 144(4), 415-418. https://doi.org/10.1093/jb/mvn107