Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants.

Yasusi Yamamoto, Jing Leng, Jian-Ren Shen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We describe methods to isolate highly active oxygen-evolving photosystem II (PSII) membranes and core complexes from higher plants, and to purify subunits of the oxygen-evolving complex (OEC). The membrane samples used as the material for various in vitro studies of PSII are prepared by solubilizing thylakoid membranes with the nonionic detergent Triton X-100, and the core complexes are prepared by further solubilization of the PSII membranes with n-dodecyl-β-D-maltoside (β-DDM). The OEC subunit proteins are dissociated from the PSII-enriched membranes by alkaline or salt treatment, and are then purified by ion-exchange chromatography using an automated high performance liquid chromatography system.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalMethods in Molecular Biology
Volume684
Publication statusPublished - 2011
Externally publishedYes

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Plant Proteins
Photosystem II Protein Complex
Protein Subunits
Oxygen
Membranes
Thylakoids
Ion Exchange Chromatography
Octoxynol
Detergents
Reactive Oxygen Species
Salts
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants. / Yamamoto, Yasusi; Leng, Jing; Shen, Jian-Ren.

In: Methods in Molecular Biology, Vol. 684, 2011, p. 1-10.

Research output: Contribution to journalArticle

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