Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants.

Yasusi Yamamoto, Shinsuke Sakuma, Jian Ren Shen

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Methods for the isolation of highly active oxygen-evolving photosystem (PS)II membranes from higher plants and the purification of the oxygen-evolving complex (OEC) subunits are described. Membrane samples used as the material for various in vitro studies of PSII are prepared by solubilization of thylakoid membranes with the non-ionic detergent Triton X-100. The OEC subunit proteins are dissociated from the PSII-enriched membranes by alkaline treatment or salt treatment, and then purified by ion-exchange chromatography using an automated high-performance liquid chromatography system.

    Original languageEnglish
    Pages (from-to)29-36
    Number of pages8
    JournalMethods in molecular biology (Clifton, N.J.)
    Volume274
    Publication statusPublished - 2004

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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