Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants.

Yasusi Yamamoto, Shinsuke Sakuma, Jian-Ren Shen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Methods for the isolation of highly active oxygen-evolving photosystem (PS)II membranes from higher plants and the purification of the oxygen-evolving complex (OEC) subunits are described. Membrane samples used as the material for various in vitro studies of PSII are prepared by solubilization of thylakoid membranes with the non-ionic detergent Triton X-100. The OEC subunit proteins are dissociated from the PSII-enriched membranes by alkaline treatment or salt treatment, and then purified by ion-exchange chromatography using an automated high-performance liquid chromatography system.

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalMethods in Molecular Biology
Volume274
Publication statusPublished - 2004

Fingerprint

Plant Proteins
Photosystem II Protein Complex
Protein Subunits
Oxygen
Membranes
Thylakoids
Ion Exchange Chromatography
Octoxynol
Detergents
Reactive Oxygen Species
Salts
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants. / Yamamoto, Yasusi; Sakuma, Shinsuke; Shen, Jian-Ren.

In: Methods in Molecular Biology, Vol. 274, 2004, p. 29-36.

Research output: Contribution to journalArticle

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