Isolation of P1cinC(+) and P1cinC(-) mutants and detection of their polypeptides involved in host specificity

Akira Tominaga, Kiyoshi Nakamura, Masatoshi Enomoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

P1cinC(+) mutants were isolated from a P1CMclrl00 lysogen based on the criterion that the cin mutation cannot restore flagellar phase variation of the hin pin strain, and a P1cinC(-) mutant was isolated from a P1cinC (+) lysogen of the hin+ pin+ strain by utilizing the difference in colonial types at 41°C. The hin+ pin+/P1cinC(-) strain yielded P1cinC(+) lysogens at a rate of 10-2 per bacterium while the hin pin/P1cinC(-) strain was considerably stable. Anti-P1cinC(+) serum neutralized both the P1cinC(-) and MuG(+) particles to 16% the rate of the homologous combination whereas anti-P1cinC(-) serum neutralized the P1cinC(+) particles to 45% the rate of the homologous combination. This indicates that P1cinC(+) and P1cinC(-) bear antigenically distinct polypeptides concerned with host specificity and that P1cinC(+) shares some antigen(s) involved in infectivity with MuG(+). The analysis of whole phage proteins revealed that P1cinC(+) had a 105 kd polypeptide, and P1cinC(-) had 101 kd instead of 105 kd.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalThe Japanese Journal of Genetics
Volume61
Issue number1
DOIs
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Genetics

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