Isolation of mutants with aberrant mitochondrial morphology from Arabidopsis thaliana

Xiaoge Feng, Shin Ichi Arimura, Hiro Yuki Hirano, Wataru Sakamoto, Nobuhiro Tsutsumi

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

To identify genes related to plant mitochondrial morphology and dynamics, novel mutants with respect to mitochondrial morphology were isolated from an ethyl methane sulphonate (EMS)-mutated population of Arabidopsis thaliana. Mitochondria were visualized by transforming Arabidopsis with a gene for a fusion protein consisting of GFP and a mitochondria-targeting pre-sequence. From 19,000 M2 populations, 17 mutants were isolated by fluorescent microscopic observations. All mitochondria in these mutants were longer and/or larger than wild-type mitochondria. The approximate chromosomal loci of the mutations of seven mutants that grew well were determined. The mitochondrial phenotypes of six of the mutants were recessive but the mitochondrial phenotype of the seventh mutant was dominant. Chromosomal rough mapping of the seven mutants showed that the mutations occurred at four different loci. At least one of these loci was novel, i.e., it was different from loci of other known mitochondrial morphology mutants of Arabidopsis and different from loci of Arabidopsis homologues of yeast genes related to mitochondrial morphology.

Original languageEnglish
Pages (from-to)301-305
Number of pages5
JournalGenes and Genetic Systems
Volume79
Issue number5
DOIs
Publication statusPublished - Oct 2004

Keywords

  • Arabidopsis thaliana
  • Chromosomal mapping
  • Mitochondrial fission
  • Mitochondrial morphology mutants

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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