TY - JOUR
T1 - Isolation of mutants showing temperature-sensitive cell growth from embryonal carcinoma cells
T2 - Control of stem cell differentiation by incubation temperatures
AU - Nishimune, Yoshitake
AU - Nishina, Yukio
AU - Sumi, Tetsuro
AU - Kosaka, Mitsuko
AU - Takeda, Masashi
AU - Matsumoto, Keishi
AU - Matsushiro, Aizo
AU - Sakuda, Masayoshi
PY - 1989/11/30
Y1 - 1989/11/30
N2 - Embryonal carcinoma(EC) cells, the undifferentiated stem cells of teratocarcinomas, have many properties in common with pluripotent embryonic cells, and thus provide an excellent system for studying the early events involved in embryonic development and stem cell differentiation. We have isolated three novel mutants with temperature-sensitive (ts) cell growth that were able to differentiate at a non-permissive temperature for cell growth. These mutations affect the progression of the cell cycle, leading to the transient accumulation of cells in a specific phase, the S phase, of the cell cycle, which is likely to be the primary cause of stem cell differentiation of EC cells at non-permissive temperature. Isolation of these mutants strongly supports the notion that there is a close association between the inhibition of DNA synthesis and EC cell differentiation.
AB - Embryonal carcinoma(EC) cells, the undifferentiated stem cells of teratocarcinomas, have many properties in common with pluripotent embryonic cells, and thus provide an excellent system for studying the early events involved in embryonic development and stem cell differentiation. We have isolated three novel mutants with temperature-sensitive (ts) cell growth that were able to differentiate at a non-permissive temperature for cell growth. These mutations affect the progression of the cell cycle, leading to the transient accumulation of cells in a specific phase, the S phase, of the cell cycle, which is likely to be the primary cause of stem cell differentiation of EC cells at non-permissive temperature. Isolation of these mutants strongly supports the notion that there is a close association between the inhibition of DNA synthesis and EC cell differentiation.
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U2 - 10.1016/0006-291X(89)91034-6
DO - 10.1016/0006-291X(89)91034-6
M3 - Article
C2 - 2574037
AN - SCOPUS:0024380921
VL - 165
SP - 65
EP - 72
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -