Isolation of mutants showing temperature-sensitive cell growth from embryonal carcinoma cells

Control of stem cell differentiation by incubation temperatures

Yoshitake Nishimune, Yukio Nishina, Tetsuro Sumi, Mitsuko Kosaka, Masashi Takeda, Keishi Matsumoto, Aizo Matsushiro, Masayoshi Sakuda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Embryonal carcinoma(EC) cells, the undifferentiated stem cells of teratocarcinomas, have many properties in common with pluripotent embryonic cells, and thus provide an excellent system for studying the early events involved in embryonic development and stem cell differentiation. We have isolated three novel mutants with temperature-sensitive (ts) cell growth that were able to differentiate at a non-permissive temperature for cell growth. These mutations affect the progression of the cell cycle, leading to the transient accumulation of cells in a specific phase, the S phase, of the cell cycle, which is likely to be the primary cause of stem cell differentiation of EC cells at non-permissive temperature. Isolation of these mutants strongly supports the notion that there is a close association between the inhibition of DNA synthesis and EC cell differentiation.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume165
Issue number1
DOIs
Publication statusPublished - Nov 30 1989
Externally publishedYes

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Embryonal Carcinoma Stem Cells
Cell growth
Stem cells
Cell Differentiation
Stem Cells
Cells
Temperature
Growth
Cell Cycle
Embryonic Stem Cells
S Phase
Embryonic Development
Association reactions
Mutation
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation of mutants showing temperature-sensitive cell growth from embryonal carcinoma cells : Control of stem cell differentiation by incubation temperatures. / Nishimune, Yoshitake; Nishina, Yukio; Sumi, Tetsuro; Kosaka, Mitsuko; Takeda, Masashi; Matsumoto, Keishi; Matsushiro, Aizo; Sakuda, Masayoshi.

In: Biochemical and Biophysical Research Communications, Vol. 165, No. 1, 30.11.1989, p. 65-72.

Research output: Contribution to journalArticle

Nishimune, Yoshitake ; Nishina, Yukio ; Sumi, Tetsuro ; Kosaka, Mitsuko ; Takeda, Masashi ; Matsumoto, Keishi ; Matsushiro, Aizo ; Sakuda, Masayoshi. / Isolation of mutants showing temperature-sensitive cell growth from embryonal carcinoma cells : Control of stem cell differentiation by incubation temperatures. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 165, No. 1. pp. 65-72.
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