Isolation of lactic acid bacteria bound to the porcine intestinal mucosa and an analysis of their moonlighting adhesins

Hideki Kinoshita, Satoko Ohuchi, Kensuke Arakawa, Masamichi Watanabe, Haruki Kitazawa, Tadao Saito

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The adhesion of lactic acid bacteria (LAB) to the intestinal mucosa is one of the criteria in selecting for probiotics. Eighteen LAB were isolated from porcine intestinal mucin (PIM): ten strains of Lactobacillus, six strains of Weissella, and two strains of Streptococcus. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for phosphate-buffered saline (PBS) extracts from the LAB, many bands were detected in half of the samples, while a few and/or no clear bands were detected in the other half. All six of the selected LAB showed adhesion to PIM. L. johnsonii MYU 214 and MYU 221 showed adhesion at more than 10%. W. viridescens MYU 208, L. reuteri MYU 213, L. mucosae MYU 225, and L. agilis MYU 227 showed medium levels of adhesion at 5.9-8.3%. In a comprehensive analysis for the adhesins in the PBS extracts using a receptor overlay analysis, many moonlighting proteins were detected and identified as candidates for adhesins: GroEL, enolase, and elongation factor Tu in MYU 208; peptidase C1, enolase, formyl-CoA transferase, phosphoglyceromutase, triosephosphate isomerase, and phosphofructokinase in MYU 221; and DnaK, enolase, and phosphoglycerate kinase in MYU 227. These proteins in the PBS extracts, which included such things as molecular chaperones and glycolytic enzymes, may play important roles as adhesins.

Original languageEnglish
Pages (from-to)185-196
Number of pages12
JournalBioscience of Microbiota, Food and Health
Volume35
Issue number4
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

adhesins
Intestinal Mucosa
intestinal mucosa
phosphopyruvate hydratase
lactic acid bacteria
adhesion
Phosphopyruvate Hydratase
Lactic Acid
Swine
Bacteria
swine
mucins
Phosphates
Mucins
phosphates
extracts
Weissella
CoA-transferases
Phosphoglycerate Mutase
Peptide Elongation Factor Tu

Keywords

  • Adhesin
  • Adhesion
  • Lactic acid bacteria
  • Moonlighting protein
  • Porcine intestinal mucin
  • Probiotics

ASJC Scopus subject areas

  • Food Science
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Gastroenterology

Cite this

Isolation of lactic acid bacteria bound to the porcine intestinal mucosa and an analysis of their moonlighting adhesins. / Kinoshita, Hideki; Ohuchi, Satoko; Arakawa, Kensuke; Watanabe, Masamichi; Kitazawa, Haruki; Saito, Tadao.

In: Bioscience of Microbiota, Food and Health, Vol. 35, No. 4, 01.01.2016, p. 185-196.

Research output: Contribution to journalArticle

Kinoshita, Hideki ; Ohuchi, Satoko ; Arakawa, Kensuke ; Watanabe, Masamichi ; Kitazawa, Haruki ; Saito, Tadao. / Isolation of lactic acid bacteria bound to the porcine intestinal mucosa and an analysis of their moonlighting adhesins. In: Bioscience of Microbiota, Food and Health. 2016 ; Vol. 35, No. 4. pp. 185-196.
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