Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method

Kripamoy Aguan, Kazuyuki Sugawara, Nobuhiro Suzuki, Tomonobu Kusano

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We have isolated cDNAs that correspond to three distinct low-temperature-induced mRNAs from rice cells using an effective, simple subtraction method. The corresponding genes were designated lip (gene encoding low-temperature-induced protein) -5,-9 and -19. The expression of these genes was slightly stimulated lip5 and lip19) or unaffected (lip9) by abscisic acid, unlike the expression of the rabl6A-D genes which are readily induced by either abscisic acid or high osmotic stress. Time-course analysis revealed that while the lip5 gene was induced 3 h after temperature down-shift, and the lip9 and lipl9 genes were induced 6 h after such a shift.

Original languageEnglish
Pages (from-to)1285-1289
Number of pages5
JournalPlant and Cell Physiology
Volume32
Issue number8
Publication statusPublished - Dec 1 1991
Externally publishedYes

Keywords

  • Abscisic acid
  • CDNA subtraction
  • Low temperature
  • Oryza sativa

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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