Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method

Kripamoy Aguan, Kazuyuki Sugawara, Nobuhiro Suzuki, Tomonobu Kusano

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We have isolated cDNAs that correspond to three distinct low-temperature-induced mRNAs from rice cells using an effective, simple subtraction method. The corresponding genes were designated lip (gene encoding low-temperature-induced protein) -5,-9 and -19. The expression of these genes was slightly stimulated lip5 and lip19) or unaffected (lip9) by abscisic acid, unlike the expression of the rabl6A-D genes which are readily induced by either abscisic acid or high osmotic stress. Time-course analysis revealed that while the lip5 gene was induced 3 h after temperature down-shift, and the lip9 and lipl9 genes were induced 6 h after such a shift.

Original languageEnglish
Pages (from-to)1285-1289
Number of pages5
JournalPlant and Cell Physiology
Volume32
Issue number8
Publication statusPublished - Dec 1991
Externally publishedYes

Fingerprint

rice protein
Isolation
rice
Genes
Gene
Proteins
Protein
Temperature
protein
gene
Abscisic Acid
abscisic acid
temperature
genes
methodology
Osmotic Stress
Gene encoding
Acids
Osmotic Pressure
osmotic stress

Keywords

  • Abscisic acid
  • CDNA subtraction
  • Low temperature
  • Oryza sativa

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method. / Aguan, Kripamoy; Sugawara, Kazuyuki; Suzuki, Nobuhiro; Kusano, Tomonobu.

In: Plant and Cell Physiology, Vol. 32, No. 8, 12.1991, p. 1285-1289.

Research output: Contribution to journalArticle

Aguan, Kripamoy ; Sugawara, Kazuyuki ; Suzuki, Nobuhiro ; Kusano, Tomonobu. / Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method. In: Plant and Cell Physiology. 1991 ; Vol. 32, No. 8. pp. 1285-1289.
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