Isolation of cDNAs coding for NtEPb1-b3, marker proteins for pollen dedifferentiation in a tobacco pollen culture system

Masaharu Kyo, Naoki Yamaji, Yasukazu Yuasa, Tsuyoshi Maeda, Hiroshi Fukui

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Several phosphoproteins, Nicotiana tabacum L. embryogenic pollen-abundant phosphoproteins (NtEPs), characteristically appear in the dedifferentiation process from immature pollen grains to embryogenic cells in a pollen culture system. Among NtEPs we focused our attention on three proteins (NtEPb1-b3) which showed the highest correlation with the dedifferentiation and possessed different pI values and similar molecular weights (ca. 22 kDa). Using probes designed from the N-terminal amino acid sequence common to the three, we isolated 14 clones of cDNA belonging to three similar sequences which probably correspond to NtEPb1-b3. The predicted amino acid sequences showed moderate homology to NtEPc, several type-1 copper-binding glycoproteins and a kind of early nodulin. The level of the transcripts for NtEPbs is highly associated with the pollen dedifferentiation but not with pollen maturation nor with cell division accompanied by meiosis or proliferation of BY-2 cells. Such an expression manner was distinguished from that of a gene coding for A-type cyclin (Ntcyc 25), indicating that NtEPb genes are not under cell cycle control. These results suggest that there exist genes related to an unknown event other than the reentrance of cell cycle in the dedifferentiation process of immature pollen that may be important for acquisition of embryogenic competence.

Original languageEnglish
Pages (from-to)1055-1061
Number of pages7
JournalPlant Science
Volume163
Issue number5
DOIs
Publication statusPublished - Nov 1 2002
Externally publishedYes

Fingerprint

Tobacco
Phosphoproteins
Pollen
tobacco
Complementary DNA
Genes
Cells
pollen
phosphoproteins
Amino Acids
Cyclin A
Proteins
Copper
Glycoproteins
Nicotiana tabacum
Molecular weight
Amino Acid Sequence
cell cycle
amino acid sequences
immatures

Keywords

  • Dedifferentiation
  • Nicotiana tabacum L.
  • Phosphoproteins
  • Pollen embryogenesis

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Isolation of cDNAs coding for NtEPb1-b3, marker proteins for pollen dedifferentiation in a tobacco pollen culture system. / Kyo, Masaharu; Yamaji, Naoki; Yuasa, Yasukazu; Maeda, Tsuyoshi; Fukui, Hiroshi.

In: Plant Science, Vol. 163, No. 5, 01.11.2002, p. 1055-1061.

Research output: Contribution to journalArticle

Kyo, Masaharu ; Yamaji, Naoki ; Yuasa, Yasukazu ; Maeda, Tsuyoshi ; Fukui, Hiroshi. / Isolation of cDNAs coding for NtEPb1-b3, marker proteins for pollen dedifferentiation in a tobacco pollen culture system. In: Plant Science. 2002 ; Vol. 163, No. 5. pp. 1055-1061.
@article{fb5a04dbab0249b28ef814de8c72da7f,
title = "Isolation of cDNAs coding for NtEPb1-b3, marker proteins for pollen dedifferentiation in a tobacco pollen culture system",
abstract = "Several phosphoproteins, Nicotiana tabacum L. embryogenic pollen-abundant phosphoproteins (NtEPs), characteristically appear in the dedifferentiation process from immature pollen grains to embryogenic cells in a pollen culture system. Among NtEPs we focused our attention on three proteins (NtEPb1-b3) which showed the highest correlation with the dedifferentiation and possessed different pI values and similar molecular weights (ca. 22 kDa). Using probes designed from the N-terminal amino acid sequence common to the three, we isolated 14 clones of cDNA belonging to three similar sequences which probably correspond to NtEPb1-b3. The predicted amino acid sequences showed moderate homology to NtEPc, several type-1 copper-binding glycoproteins and a kind of early nodulin. The level of the transcripts for NtEPbs is highly associated with the pollen dedifferentiation but not with pollen maturation nor with cell division accompanied by meiosis or proliferation of BY-2 cells. Such an expression manner was distinguished from that of a gene coding for A-type cyclin (Ntcyc 25), indicating that NtEPb genes are not under cell cycle control. These results suggest that there exist genes related to an unknown event other than the reentrance of cell cycle in the dedifferentiation process of immature pollen that may be important for acquisition of embryogenic competence.",
keywords = "Dedifferentiation, Nicotiana tabacum L., Phosphoproteins, Pollen embryogenesis",
author = "Masaharu Kyo and Naoki Yamaji and Yasukazu Yuasa and Tsuyoshi Maeda and Hiroshi Fukui",
year = "2002",
month = "11",
day = "1",
doi = "10.1016/S0168-9452(02)00280-7",
language = "English",
volume = "163",
pages = "1055--1061",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "5",

}

TY - JOUR

T1 - Isolation of cDNAs coding for NtEPb1-b3, marker proteins for pollen dedifferentiation in a tobacco pollen culture system

AU - Kyo, Masaharu

AU - Yamaji, Naoki

AU - Yuasa, Yasukazu

AU - Maeda, Tsuyoshi

AU - Fukui, Hiroshi

PY - 2002/11/1

Y1 - 2002/11/1

N2 - Several phosphoproteins, Nicotiana tabacum L. embryogenic pollen-abundant phosphoproteins (NtEPs), characteristically appear in the dedifferentiation process from immature pollen grains to embryogenic cells in a pollen culture system. Among NtEPs we focused our attention on three proteins (NtEPb1-b3) which showed the highest correlation with the dedifferentiation and possessed different pI values and similar molecular weights (ca. 22 kDa). Using probes designed from the N-terminal amino acid sequence common to the three, we isolated 14 clones of cDNA belonging to three similar sequences which probably correspond to NtEPb1-b3. The predicted amino acid sequences showed moderate homology to NtEPc, several type-1 copper-binding glycoproteins and a kind of early nodulin. The level of the transcripts for NtEPbs is highly associated with the pollen dedifferentiation but not with pollen maturation nor with cell division accompanied by meiosis or proliferation of BY-2 cells. Such an expression manner was distinguished from that of a gene coding for A-type cyclin (Ntcyc 25), indicating that NtEPb genes are not under cell cycle control. These results suggest that there exist genes related to an unknown event other than the reentrance of cell cycle in the dedifferentiation process of immature pollen that may be important for acquisition of embryogenic competence.

AB - Several phosphoproteins, Nicotiana tabacum L. embryogenic pollen-abundant phosphoproteins (NtEPs), characteristically appear in the dedifferentiation process from immature pollen grains to embryogenic cells in a pollen culture system. Among NtEPs we focused our attention on three proteins (NtEPb1-b3) which showed the highest correlation with the dedifferentiation and possessed different pI values and similar molecular weights (ca. 22 kDa). Using probes designed from the N-terminal amino acid sequence common to the three, we isolated 14 clones of cDNA belonging to three similar sequences which probably correspond to NtEPb1-b3. The predicted amino acid sequences showed moderate homology to NtEPc, several type-1 copper-binding glycoproteins and a kind of early nodulin. The level of the transcripts for NtEPbs is highly associated with the pollen dedifferentiation but not with pollen maturation nor with cell division accompanied by meiosis or proliferation of BY-2 cells. Such an expression manner was distinguished from that of a gene coding for A-type cyclin (Ntcyc 25), indicating that NtEPb genes are not under cell cycle control. These results suggest that there exist genes related to an unknown event other than the reentrance of cell cycle in the dedifferentiation process of immature pollen that may be important for acquisition of embryogenic competence.

KW - Dedifferentiation

KW - Nicotiana tabacum L.

KW - Phosphoproteins

KW - Pollen embryogenesis

UR - http://www.scopus.com/inward/record.url?scp=0036850949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036850949&partnerID=8YFLogxK

U2 - 10.1016/S0168-9452(02)00280-7

DO - 10.1016/S0168-9452(02)00280-7

M3 - Article

AN - SCOPUS:0036850949

VL - 163

SP - 1055

EP - 1061

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 5

ER -