Isolation of bacteriophages for fastidious bacteria

Shigenobu Matsuzaki, Jumpei Uchiyama, Iyo Takemura-Uchiyama, Takako Ujihara, Masanori Daibata

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)


One of the most important factors for successful bacteriophage therapy is, undoubtedly, the isolation of excellent therapeutic candidate bacteriophages. There are only a few reports about active bacteriophages in the fastidious bacteria Helicobacter pylori. In this chapter, we describe a method for isolating and purifying KHP30-like bacteriophages in H. pylori, which have lytic and pseudolysogenic life cycles.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages8
Publication statusPublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Helicobacter pylori
  • Isolation
  • KHP30-like bacteriophage
  • Lytic and pseudolysogenic life cycles
  • Purification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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