Abstract
The yeast Abc1 protein acts as a chaperone-like protein essential for the proper conformation and efficient functioning of the respiratory complex III. By functional complementation of a yeast abc1 mutant, we have identified an Arabidopsis thaliana cDNA that corresponds to a single copy gene and encodes a protein sharing 45% similarity with the yeast Abc1p protein. Cytochrome spectra and respiratory activity measurements have shown that the plant protein allows a partial restoration of the complex III activity. No major difference in the steady-state level of ABC1At mRNA was observed in various plant tissues, suggesting that ABC1At is constitutively expressed in A. thaliana. Phylogenetic analysis revealed that the Abc1At protein belongs to a large family of proteins composed of two eukaryotic and one prokaryotic subgroups differing by their degree of similarity and probably by their function.
| Original language | English |
|---|---|
| Pages (from-to) | 117-125 |
| Number of pages | 9 |
| Journal | Gene |
| Volume | 221 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Oct 9 1998 |
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Keywords
- ABC1
- Complex III biogenesis
- Evolutionary conservation
- Mitochondria
- Saccharomyces cerevisiae
ASJC Scopus subject areas
- Genetics
Cite this
Isolation of an Arabidopsis thaliana cDNA by complementation of a yeast abc1 deletion mutant deficient in complex III respiratory activity. / Cardazzo, B.; Hamel, P.; Sakamoto, Wataru; Wintz, H.; Dujardin, G.
In: Gene, Vol. 221, No. 1, 09.10.1998, p. 117-125.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of an Arabidopsis thaliana cDNA by complementation of a yeast abc1 deletion mutant deficient in complex III respiratory activity
AU - Cardazzo, B.
AU - Hamel, P.
AU - Sakamoto, Wataru
AU - Wintz, H.
AU - Dujardin, G.
PY - 1998/10/9
Y1 - 1998/10/9
N2 - The yeast Abc1 protein acts as a chaperone-like protein essential for the proper conformation and efficient functioning of the respiratory complex III. By functional complementation of a yeast abc1 mutant, we have identified an Arabidopsis thaliana cDNA that corresponds to a single copy gene and encodes a protein sharing 45% similarity with the yeast Abc1p protein. Cytochrome spectra and respiratory activity measurements have shown that the plant protein allows a partial restoration of the complex III activity. No major difference in the steady-state level of ABC1At mRNA was observed in various plant tissues, suggesting that ABC1At is constitutively expressed in A. thaliana. Phylogenetic analysis revealed that the Abc1At protein belongs to a large family of proteins composed of two eukaryotic and one prokaryotic subgroups differing by their degree of similarity and probably by their function.
AB - The yeast Abc1 protein acts as a chaperone-like protein essential for the proper conformation and efficient functioning of the respiratory complex III. By functional complementation of a yeast abc1 mutant, we have identified an Arabidopsis thaliana cDNA that corresponds to a single copy gene and encodes a protein sharing 45% similarity with the yeast Abc1p protein. Cytochrome spectra and respiratory activity measurements have shown that the plant protein allows a partial restoration of the complex III activity. No major difference in the steady-state level of ABC1At mRNA was observed in various plant tissues, suggesting that ABC1At is constitutively expressed in A. thaliana. Phylogenetic analysis revealed that the Abc1At protein belongs to a large family of proteins composed of two eukaryotic and one prokaryotic subgroups differing by their degree of similarity and probably by their function.
KW - ABC1
KW - Complex III biogenesis
KW - Evolutionary conservation
KW - Mitochondria
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=0032500832&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032500832&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(98)00417-X
DO - 10.1016/S0378-1119(98)00417-X
M3 - Article
C2 - 9852956
AN - SCOPUS:0032500832
VL - 221
SP - 117
EP - 125
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -