Isolation of an Arabidopsis thaliana cDNA by complementation of a yeast abc1 deletion mutant deficient in complex III respiratory activity

B. Cardazzo, P. Hamel, Wataru Sakamoto, H. Wintz, G. Dujardin

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The yeast Abc1 protein acts as a chaperone-like protein essential for the proper conformation and efficient functioning of the respiratory complex III. By functional complementation of a yeast abc1 mutant, we have identified an Arabidopsis thaliana cDNA that corresponds to a single copy gene and encodes a protein sharing 45% similarity with the yeast Abc1p protein. Cytochrome spectra and respiratory activity measurements have shown that the plant protein allows a partial restoration of the complex III activity. No major difference in the steady-state level of ABC1At mRNA was observed in various plant tissues, suggesting that ABC1At is constitutively expressed in A. thaliana. Phylogenetic analysis revealed that the Abc1At protein belongs to a large family of proteins composed of two eukaryotic and one prokaryotic subgroups differing by their degree of similarity and probably by their function.

Original languageEnglish
Pages (from-to)117-125
Number of pages9
JournalGene
Volume221
Issue number1
DOIs
Publication statusPublished - Oct 9 1998

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Electron Transport Complex III
Arabidopsis
Complementary DNA
Yeasts
Fungal Proteins
Proteins
Plant Proteins
Cytochromes
Messenger RNA
Genes

Keywords

  • ABC1
  • Complex III biogenesis
  • Evolutionary conservation
  • Mitochondria
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Genetics

Cite this

Isolation of an Arabidopsis thaliana cDNA by complementation of a yeast abc1 deletion mutant deficient in complex III respiratory activity. / Cardazzo, B.; Hamel, P.; Sakamoto, Wataru; Wintz, H.; Dujardin, G.

In: Gene, Vol. 221, No. 1, 09.10.1998, p. 117-125.

Research output: Contribution to journalArticle

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