Isolation of a rice regeneration quantitative trait loci gene and its application to transformation systems

Asuka Nishimura, Motoyuki Ashikari, Shaoyang Lin, Tomonori Takashi, Enrique R. Angeles, Toshio Yamamoto, Makoto Matsuoka

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Regeneration of plant organs is often the essential step in genetic transformation; however, the regeneration ability of a plant varies depending on the genetic background. By conventional crosses of low-regeneration rice strain Koshihikari with high-regeneration rice strain Kasalath, we identified some quantitative trait loci, which control the regeneration ability in rice. Using a map-based cloning strategy, we isolated a main quantitative trait loci gene encoding ferredoxin-nitrite reductase (NiR) that determines regeneration ability in rice. Molecular analyses revealed that the poor regeneration ability of Koshihikari is caused by lower expression than in Kasalath and the specific activity of NiR. Using the NiR gene as a selection marker, we succeeded in selectively transforming a foreign gene into rice without exogenous marker genes. Our results demonstrate that nitrate assimilation is an important process in rice regeneration and also provide an additional selectable marker for rice transformation.

Original languageEnglish
Pages (from-to)11940-11944
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number33
DOIs
Publication statusPublished - Aug 16 2005
Externally publishedYes

Fingerprint

Quantitative Trait Loci
Regeneration
Aptitude
Nitrite Reductases
Ferredoxin-Nitrite Reductase
Genes
Genetic Transformation
Internal-External Control
Oryza
Nitrates
Organism Cloning

Keywords

  • Ferredoxin-nitrate reductase
  • Regeneration ability
  • Selectable marker

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Isolation of a rice regeneration quantitative trait loci gene and its application to transformation systems. / Nishimura, Asuka; Ashikari, Motoyuki; Lin, Shaoyang; Takashi, Tomonori; Angeles, Enrique R.; Yamamoto, Toshio; Matsuoka, Makoto.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 33, 16.08.2005, p. 11940-11944.

Research output: Contribution to journalArticle

Nishimura, Asuka ; Ashikari, Motoyuki ; Lin, Shaoyang ; Takashi, Tomonori ; Angeles, Enrique R. ; Yamamoto, Toshio ; Matsuoka, Makoto. / Isolation of a rice regeneration quantitative trait loci gene and its application to transformation systems. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 33. pp. 11940-11944.
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