Isolation of a novel lateral-rootless mutant in rice (Oryza sativa L.) with reduced sensitivity to auxin

Hua Wang, Shin Taketa, Akio Miyao, Hirohiko Hirochika, Masahiko Ichii

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Lateral roots play an important role in the acquisition of nutrients and anchorage of the whole plant. To better understand the mechanisms underlying lateral root development, we isolated a new lateral-rootless mutant lrt2 in screening for 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in M2 lines of rice (Oryza sativa L. cv. Nipponbare) generated by tissue culture. lrt2 failed to form lateral roots and exhibited altered root response to gravity. Analysis for auxin resistance showed that lrt2 was less sensitive to various auxins including 2,4-D, indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA) compared with wild type, but was similarly sensitive to auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). This suggests that the reduced sensitivity to auxin in lrt2 might be caused by a disruption in auxin response rather than in auxin transport. Genetic analysis indicated that the lateral-rootless phenotype of lrt2 is due to a recessive mutation. To map the lrt2 gene, we tested molecular markers by bulk segregant analysis. The lrt2 gene was localized to a 10.8 cM interval on the short arm of chromosome 2, flanked by two sequence-tagged site (STS) markers Lrt2P1 and Lrt2P2.

Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalPlant Science
Volume170
Issue number1
DOIs
Publication statusPublished - Jan 2006
Externally publishedYes

Fingerprint

Indoleacetic Acids
auxins
Oryza sativa
rice
mutants
2,4-Dichlorophenoxyacetic Acid
2,4-D
Genes
Sequence Tagged Sites
naphthylphthalamic acid
Tissue culture
Chromosomes, Human, Pair 2
Gravitation
Chromosomes
indole butyric acid
gravity
naphthaleneacetic acid
indole acetic acid
Nutrients
tissue culture

Keywords

  • Auxin resistance
  • Gravitropism
  • Lateral root formation
  • lrt2
  • Rice
  • STS markers

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Isolation of a novel lateral-rootless mutant in rice (Oryza sativa L.) with reduced sensitivity to auxin. / Wang, Hua; Taketa, Shin; Miyao, Akio; Hirochika, Hirohiko; Ichii, Masahiko.

In: Plant Science, Vol. 170, No. 1, 01.2006, p. 70-77.

Research output: Contribution to journalArticle

Wang, Hua ; Taketa, Shin ; Miyao, Akio ; Hirochika, Hirohiko ; Ichii, Masahiko. / Isolation of a novel lateral-rootless mutant in rice (Oryza sativa L.) with reduced sensitivity to auxin. In: Plant Science. 2006 ; Vol. 170, No. 1. pp. 70-77.
@article{9c6d51dc2d8747adbd76928cd2547d04,
title = "Isolation of a novel lateral-rootless mutant in rice (Oryza sativa L.) with reduced sensitivity to auxin",
abstract = "Lateral roots play an important role in the acquisition of nutrients and anchorage of the whole plant. To better understand the mechanisms underlying lateral root development, we isolated a new lateral-rootless mutant lrt2 in screening for 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in M2 lines of rice (Oryza sativa L. cv. Nipponbare) generated by tissue culture. lrt2 failed to form lateral roots and exhibited altered root response to gravity. Analysis for auxin resistance showed that lrt2 was less sensitive to various auxins including 2,4-D, indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA) compared with wild type, but was similarly sensitive to auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). This suggests that the reduced sensitivity to auxin in lrt2 might be caused by a disruption in auxin response rather than in auxin transport. Genetic analysis indicated that the lateral-rootless phenotype of lrt2 is due to a recessive mutation. To map the lrt2 gene, we tested molecular markers by bulk segregant analysis. The lrt2 gene was localized to a 10.8 cM interval on the short arm of chromosome 2, flanked by two sequence-tagged site (STS) markers Lrt2P1 and Lrt2P2.",
keywords = "Auxin resistance, Gravitropism, Lateral root formation, lrt2, Rice, STS markers",
author = "Hua Wang and Shin Taketa and Akio Miyao and Hirohiko Hirochika and Masahiko Ichii",
year = "2006",
month = "1",
doi = "10.1016/j.plantsci.2005.08.005",
language = "English",
volume = "170",
pages = "70--77",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Isolation of a novel lateral-rootless mutant in rice (Oryza sativa L.) with reduced sensitivity to auxin

AU - Wang, Hua

AU - Taketa, Shin

AU - Miyao, Akio

AU - Hirochika, Hirohiko

AU - Ichii, Masahiko

PY - 2006/1

Y1 - 2006/1

N2 - Lateral roots play an important role in the acquisition of nutrients and anchorage of the whole plant. To better understand the mechanisms underlying lateral root development, we isolated a new lateral-rootless mutant lrt2 in screening for 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in M2 lines of rice (Oryza sativa L. cv. Nipponbare) generated by tissue culture. lrt2 failed to form lateral roots and exhibited altered root response to gravity. Analysis for auxin resistance showed that lrt2 was less sensitive to various auxins including 2,4-D, indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA) compared with wild type, but was similarly sensitive to auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). This suggests that the reduced sensitivity to auxin in lrt2 might be caused by a disruption in auxin response rather than in auxin transport. Genetic analysis indicated that the lateral-rootless phenotype of lrt2 is due to a recessive mutation. To map the lrt2 gene, we tested molecular markers by bulk segregant analysis. The lrt2 gene was localized to a 10.8 cM interval on the short arm of chromosome 2, flanked by two sequence-tagged site (STS) markers Lrt2P1 and Lrt2P2.

AB - Lateral roots play an important role in the acquisition of nutrients and anchorage of the whole plant. To better understand the mechanisms underlying lateral root development, we isolated a new lateral-rootless mutant lrt2 in screening for 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in M2 lines of rice (Oryza sativa L. cv. Nipponbare) generated by tissue culture. lrt2 failed to form lateral roots and exhibited altered root response to gravity. Analysis for auxin resistance showed that lrt2 was less sensitive to various auxins including 2,4-D, indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA) compared with wild type, but was similarly sensitive to auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). This suggests that the reduced sensitivity to auxin in lrt2 might be caused by a disruption in auxin response rather than in auxin transport. Genetic analysis indicated that the lateral-rootless phenotype of lrt2 is due to a recessive mutation. To map the lrt2 gene, we tested molecular markers by bulk segregant analysis. The lrt2 gene was localized to a 10.8 cM interval on the short arm of chromosome 2, flanked by two sequence-tagged site (STS) markers Lrt2P1 and Lrt2P2.

KW - Auxin resistance

KW - Gravitropism

KW - Lateral root formation

KW - lrt2

KW - Rice

KW - STS markers

UR - http://www.scopus.com/inward/record.url?scp=27144455362&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27144455362&partnerID=8YFLogxK

U2 - 10.1016/j.plantsci.2005.08.005

DO - 10.1016/j.plantsci.2005.08.005

M3 - Article

VL - 170

SP - 70

EP - 77

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 1

ER -