Isolation of a novel isozyme of tobacco BY-2 chitinase induced by a fungal elicitor

Tomonori Shinya, Kazunari Hanai, Mikako Saito, Hideaki Matsuoka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Three isozymes of chitinase were induced by a fungal elicitor in cultured tobacco BY-2 cells. The most acidic one, designated TBC-3, was described here. The molecular mass of TBC-3 was estimated to be 28.5 kDa. The N-terminal amino acid sequence of TBC-3 was analyzed and a homology search was performed. Fifteen amino acids at the N-terminus showed 60% homology to class II chitinases from other plants but not higher than 27% homology to already known chitinases from tobacco. Therefore, TBC-3 is thought to be a novel tobacco chitinase that may be classified into class II.

Original languageEnglish
Pages (from-to)155-158
Number of pages4
JournalPlant Biotechnology
Volume21
Issue number2
DOIs
Publication statusPublished - Jun 2004
Externally publishedYes

Keywords

  • Alternaria alternata
  • Amino acid sequence homology
  • Chitinase isozyme
  • Tobacco BY-2 cells

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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