Isolation of a Novel Endophytic Bacillus Strain Capable of Transforming Pentachlorophenol and Structure Determination of Pentachlorophenol Phosphate Using Single-Crystal X-ray Diffraction

Koji Ito, Ryota Kataoka, Shunki Katayama, Hiromasa Kiyota, Ahmad Mahmood, Takashi Kikuchi, Takashi Sato, Futa Sakakibara, Kazuhiro Takagi

Research output: Contribution to journalArticlepeer-review

Abstract

A novel approach for the remediation of upland soils contaminated with pentachlorophenol (C6HCl5O; PCP) (1), a fungicide, wood perservative, and herbicide, through the exploitation of plant-endophytic bacteria may overcome the existing issues in bioaugmentaion and phytoremidiation. In this study, we isolated the endophytic Bacillus sp. strain PCP15 and determined its metabolite of PCP (1). This strain degraded 8.03 μmol L–1 PCP (1) within 24 h and generated the novel metabolite PCP phosphate (3). The PCP15 strain showed nearly complete growth inhibition of 20 μmol L−1 PCP (1). In contrast, PCP15 showed resistance to PCP phosphate (3), indicating that the phosphorylation of PCP, which has never previously been reported in organisms, contributed to the detoxification of PCP (1) in bacterial cells. Our results show the potential for practical application of this strain in hybrid remediation of PCP (1)-contaminated soils and reveal a novel PCP (1) detoxification mechanism in organisms.

Original languageEnglish
Pages (from-to)770-776
Number of pages7
JournalJournal of agricultural and food chemistry
Volume70
Issue number3
DOIs
Publication statusPublished - Jan 26 2022

Keywords

  • bioremediation
  • endophytic bacteria
  • metabolic pathway
  • PCP phosphate
  • SCX

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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