Isolation of a new minor protein (ovofactor-1), which has a cell growth promoting activity, from hen’s egg white by heparin affinity chromatography

Tadashi Nakamura, Tadao Saito, Haruki Kitazawa, Sakae Takeuchi, Takatoshi Itoh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A proteinaceous component eluted at the concentration of 0.8–1.0M NaCl was obtained from hen egg white by heparin affinity chromatography. On SDS–PAGE, the component was resolved into three bands of 18.5, 16.5, and 16.0 kDa. The isoelectric point was estimated to be pH 8.8. This stimulated the DNA synthesis and proliferation of the cultured cells from chichen embryos. The amino acid composition and N-terminal analyses of the main 18.5 kDa component showed that this is a novel minor protein in egg white, and therefore was named “Ovofactor-1”.

Original languageEnglish
Pages (from-to)1946-1948
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume59
Issue number10
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Affinity chromatography
Egg Proteins
Egg White
Isoelectric Point
Cell growth
heparin
egg albumen
affinity chromatography
Affinity Chromatography
Heparin
Amino acids
hens
cell growth
Cultured Cells
DNA
Embryonic Structures
Cells
Proteins
Amino Acids
isoelectric point

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Organic Chemistry
  • Analytical Chemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Food Science

Cite this

Isolation of a new minor protein (ovofactor-1), which has a cell growth promoting activity, from hen’s egg white by heparin affinity chromatography. / Nakamura, Tadashi; Saito, Tadao; Kitazawa, Haruki; Takeuchi, Sakae; Itoh, Takatoshi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 59, No. 10, 1995, p. 1946-1948.

Research output: Contribution to journalArticle

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AU - Itoh, Takatoshi

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