Isolation of a New Minor Protein (Ovofactor-1), Which Has a Cell Growth Promoting Activity, from Hen's Egg White by Heparin Affinity Chromatography

Tadashi Nakamura, Tadao Saito, Haruki Kitazawa, Sanae Takeuchi, Takatoshi Itoh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A proteinaceous component eluted at the concentration of 0.8-1.0 M NaCl was obtained from hen egg white by heparin affinity chromatography. On SDS-PAGE, the component was resolved into three bands of 18. 5, 16. 5, and 16.0 kDa. The isoelectric point was estimated to be pH 8.8. This stimulated the DNA synthesis and proliferation of the cultured cells from chichen embryos. The amino acid composition and N-terminal analyses of the main 18.5 kDa component showed that this is a novel minor protein in egg white, and therefore was named “Ovofactor-1”.

Original languageEnglish
Pages (from-to)1946-1948
Number of pages3
JournalBioscience, biotechnology, and biochemistry
Volume59
Issue number10
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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