Abstract
Rheumatoid arthritis (RA) is a complex disease including autoimmune disorder and resulting in inflammation. It also shows progressive proliferation of synoviocytes, and these synoviocytes destroys articular structure. In order to understand the mechanism of this abnormal proliferation of RA-originated synoviocytes in molecular level, we analyzed the gene expression profiles by using DNA chips that contain more than 10,000 genes. Comparing the expression profiles of normal and RA-originated synoviocytes, we found several genes that are highly expressed in RA-originated synoviocytes than normal synoviocytes. Among these genes, we focused on one hypothetical protein, cDNA of which contains one reading frame in its DNA fragment, indicating that this fragment is a part of large mRNA structure. The expression of this gene in RA-originated synoviocytes is about three times higher than that in normal synoviocytes by DNA chip analysis. After cDNA cloning of this mRNA, we found that the gene is identical with lumican, which encodes a collagen binding, extracellular matrix protein. This mRNA was widely distributed in many tissues but its alternatively spliced forms are differently expressed in various tissues.
| Original language | English |
|---|---|
| Pages (from-to) | 125-130 |
| Number of pages | 6 |
| Journal | Journal of Hard Tissue Biology |
| Volume | 17 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2008 |
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Keywords
- Dna chip
- Lumican
- Rheumatoid arthritis
- Synovial cells
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Biomaterials
- Medicine (miscellaneous)
- Orthopedics and Sports Medicine
- Dentistry(all)
Cite this
Isolation of a mRNA preferentially expressed in synoviocytes from rheumatoid arthritis that is identical with lumican, which encodes a collagen binding Extracellular Matrix Protein. / Mori, Hiroki; Nishida, Keiichiro; Ozaki, Toshihumi; Inoue, Hajime; Nakanishi, Tohru.
In: Journal of Hard Tissue Biology, Vol. 17, No. 3, 2008, p. 125-130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of a mRNA preferentially expressed in synoviocytes from rheumatoid arthritis that is identical with lumican, which encodes a collagen binding Extracellular Matrix Protein
AU - Mori, Hiroki
AU - Nishida, Keiichiro
AU - Ozaki, Toshihumi
AU - Inoue, Hajime
AU - Nakanishi, Tohru
PY - 2008
Y1 - 2008
N2 - Rheumatoid arthritis (RA) is a complex disease including autoimmune disorder and resulting in inflammation. It also shows progressive proliferation of synoviocytes, and these synoviocytes destroys articular structure. In order to understand the mechanism of this abnormal proliferation of RA-originated synoviocytes in molecular level, we analyzed the gene expression profiles by using DNA chips that contain more than 10,000 genes. Comparing the expression profiles of normal and RA-originated synoviocytes, we found several genes that are highly expressed in RA-originated synoviocytes than normal synoviocytes. Among these genes, we focused on one hypothetical protein, cDNA of which contains one reading frame in its DNA fragment, indicating that this fragment is a part of large mRNA structure. The expression of this gene in RA-originated synoviocytes is about three times higher than that in normal synoviocytes by DNA chip analysis. After cDNA cloning of this mRNA, we found that the gene is identical with lumican, which encodes a collagen binding, extracellular matrix protein. This mRNA was widely distributed in many tissues but its alternatively spliced forms are differently expressed in various tissues.
AB - Rheumatoid arthritis (RA) is a complex disease including autoimmune disorder and resulting in inflammation. It also shows progressive proliferation of synoviocytes, and these synoviocytes destroys articular structure. In order to understand the mechanism of this abnormal proliferation of RA-originated synoviocytes in molecular level, we analyzed the gene expression profiles by using DNA chips that contain more than 10,000 genes. Comparing the expression profiles of normal and RA-originated synoviocytes, we found several genes that are highly expressed in RA-originated synoviocytes than normal synoviocytes. Among these genes, we focused on one hypothetical protein, cDNA of which contains one reading frame in its DNA fragment, indicating that this fragment is a part of large mRNA structure. The expression of this gene in RA-originated synoviocytes is about three times higher than that in normal synoviocytes by DNA chip analysis. After cDNA cloning of this mRNA, we found that the gene is identical with lumican, which encodes a collagen binding, extracellular matrix protein. This mRNA was widely distributed in many tissues but its alternatively spliced forms are differently expressed in various tissues.
KW - Dna chip
KW - Lumican
KW - Rheumatoid arthritis
KW - Synovial cells
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U2 - 10.2485/jhtb.17.125
DO - 10.2485/jhtb.17.125
M3 - Article
VL - 17
SP - 125
EP - 130
JO - Journal of Hard Tissue Biology
JF - Journal of Hard Tissue Biology
SN - 1341-7649
IS - 3
ER -