Isolation and some properties of a strain of the iron-oxidizing bacterium Thiobacillus ferrooxidans resistant to 2,4-dinitrophenol

Tsuyoshi Sugio, Akira Fujioka, Mikio Tsuchiya, Naoki Shibusawa, Kenji Iwahori, Kazuo Kamimura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Among two hundred strains of iron-oxidizing bacteria isolated from the natural environment, only one, Thiobacillus ferrooxidans NASF-1, grew in an Fe2+ medium supplemented with 10 μM of 2,4-dinitrophenol (2,4-DNP). In contrast, growth of T. ferrooxidans AP19-3, a strain representative of iron- oxidizing bacteria sensitive to 2,4-DNP, was strongly inhibited by 2,4-DNP at 1.0 μM, and completely repressed at 10 μM. Strain NASF-1 was also resistant to an other uncoupler, carbonylcyanide m-chlorophenylhydrazone (CCCP). The iron-oxidizing activities of strains AP19-3 and NASF-1 were respectively inhibited 69 and 23% by 10 μM 2,4-DNP, while the 14CO2 uptake activities of the two strains were inhibited 31 and 5% by 2,4-DNP at 1.0 μM, and 100 and 89% at 5.0 μM. When intact cells of strain AP19-3 were incubated with Fe2+ and 1.0 μM 2,4-DNP for 10 min at pH 3.0, 49 pmol 2,4-DNP per mg of protein was incorporated into the cells. In contrast, strain NASF-1 did not incorporate 2,4-DNP under the same conditions, suggesting that, compared with strain NASF-1, strain AP19-3 has a plasma membrane through which 2,4-DNP is more easily incorporated in the cytosol.

Original languageEnglish
Pages (from-to)134-137
Number of pages4
JournalJournal of Fermentation and Bioengineering
Volume86
Issue number1
DOIs
Publication statusPublished - 1998

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Thiobacillus
2,4-Dinitrophenol
Bacteria
Iron
Cell membranes
Cytosol
Proteins
Cell Membrane

Keywords

  • 2,4- dinitrophenol
  • Iron-oxidizing bacterium
  • Thiobacillus ferrooxidans
  • Uncoupler

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Isolation and some properties of a strain of the iron-oxidizing bacterium Thiobacillus ferrooxidans resistant to 2,4-dinitrophenol. / Sugio, Tsuyoshi; Fujioka, Akira; Tsuchiya, Mikio; Shibusawa, Naoki; Iwahori, Kenji; Kamimura, Kazuo.

In: Journal of Fermentation and Bioengineering, Vol. 86, No. 1, 1998, p. 134-137.

Research output: Contribution to journalArticle

Sugio, Tsuyoshi ; Fujioka, Akira ; Tsuchiya, Mikio ; Shibusawa, Naoki ; Iwahori, Kenji ; Kamimura, Kazuo. / Isolation and some properties of a strain of the iron-oxidizing bacterium Thiobacillus ferrooxidans resistant to 2,4-dinitrophenol. In: Journal of Fermentation and Bioengineering. 1998 ; Vol. 86, No. 1. pp. 134-137.
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