Isolation and sequence of rat testis cdna for a calcium binding polypeptide similar to the regulatory subunit of calcineurin

Mikio Sugimoto, Hideki Matsui, Seiji Etoh, Tohru Shimizu, Hajime Nishio, Lizomar J.M.P. Moia, Masaaki Tokuda, Toshifumi Itano, Ikumasa Takenaka, Osamu Hatase

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Abstract

We have cloned and sequenced rat testis cDNAs coding for a calcium binding polypeptide similar to calcineurin β subunit, the Ca2+-binding subunit of the Ca2+/calmodulin stimulated protein phosphatase. Rat testis cDNA library was screened with a monoclonal antibody Va1 raised against bovine brain calcineurin β subunit. The deduced amino acid sequence is similar to that of human brain calcineurin β subunit with respect to containing four putative calcium binding sites. However, distinct differences were found: 1) The cloned cDNA had six amino acids polypeptide tail at carboxy-terminal which is absent in human brain calcineurin β subunit. This amino acids tail makes the carboxy-terminal highly hydrophilic in contrast to the human brain β subunit which is hydrophobic at carboxy-terminal; 2) eleven amino acids at the N-terminal of the cloned cDNA were completely different from the corresponding region of the brain calcineurin β subunit.

Original languageEnglish
Pages (from-to)1476-1482
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume180
Issue number3
DOIs
Publication statusPublished - Nov 14 1991

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sugimoto, M., Matsui, H., Etoh, S., Shimizu, T., Nishio, H., Moia, L. J. M. P., Tokuda, M., Itano, T., Takenaka, I., & Hatase, O. (1991). Isolation and sequence of rat testis cdna for a calcium binding polypeptide similar to the regulatory subunit of calcineurin. Biochemical and Biophysical Research Communications, 180(3), 1476-1482. https://doi.org/10.1016/S0006-291X(05)81362-2