Isolation and properties of a mutant of Escherichia coli possessing defective Na+/H+ antiporter.

Tetsuya Ishikawa, H. Hama, M. Tsuda, T. Tsuchiya

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A mutant of Escherichia coli with defective Na+/H+ antiporter was isolated. The rationale for its isolation was that cells possessing defective Na+/H+ antiporter, which is essential for establishment of a Na+ gradient, could not grow with a carbon source that was taken up with Na+. The mutant had no appreciable Na+/H+ antiporter activity, but its K+/H+ antiporter and Ca2+/H+ antiporter activities were normal. Judging from the reversion frequency, the defect seems to be due to a single mutation. The mutant could not grow at alkaline pH. Therefore, the Na+/H+ antiporter, but not the K+/H+ antiporter or the Ca2+/H+ antiporter, seems to be responsible for pH regulation in alkaline medium. This mutant will be useful for cloning the Na+/H+ antiporter gene and for detection of Na+-substrate cotransport systems.

Original languageEnglish
Pages (from-to)7443-7446
Number of pages4
JournalJournal of Biological Chemistry
Volume262
Issue number16
Publication statusPublished - Jun 5 1987
Externally publishedYes

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Sodium-Hydrogen Antiporter
Antiporters
Escherichia coli
Cell Separation
Cloning
Organism Cloning
Carbon
Genes
Defects
Mutation
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Isolation and properties of a mutant of Escherichia coli possessing defective Na+/H+ antiporter. / Ishikawa, Tetsuya; Hama, H.; Tsuda, M.; Tsuchiya, T.

In: Journal of Biological Chemistry, Vol. 262, No. 16, 05.06.1987, p. 7443-7446.

Research output: Contribution to journalArticle

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