Abstract
A mutant of Escherichia coli with defective Na+/H+ antiporter was isolated. The rationale for its isolation was that cells possessing defective Na+/H+ antiporter, which is essential for establishment of a Na+ gradient, could not grow with a carbon source that was taken up with Na+. The mutant had no appreciable Na+/H+ antiporter activity, but its K+/H+ antiporter and Ca2+/H+ antiporter activities were normal. Judging from the reversion frequency, the defect seems to be due to a single mutation. The mutant could not grow at alkaline pH. Therefore, the Na+/H+ antiporter, but not the K+/H+ antiporter or the Ca2+/H+ antiporter, seems to be responsible for pH regulation in alkaline medium. This mutant will be useful for cloning the Na+/H+ antiporter gene and for detection of Na+-substrate cotransport systems.
| Original language | English |
|---|---|
| Pages (from-to) | 7443-7446 |
| Number of pages | 4 |
| Journal | Journal of Biological Chemistry |
| Volume | 262 |
| Issue number | 16 |
| Publication status | Published - Jun 5 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Isolation and properties of a mutant of Escherichia coli possessing defective Na+/H+ antiporter. / Ishikawa, Tetsuya; Hama, H.; Tsuda, M.; Tsuchiya, T.
In: Journal of Biological Chemistry, Vol. 262, No. 16, 05.06.1987, p. 7443-7446.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and properties of a mutant of Escherichia coli possessing defective Na+/H+ antiporter.
AU - Ishikawa, Tetsuya
AU - Hama, H.
AU - Tsuda, M.
AU - Tsuchiya, T.
PY - 1987/6/5
Y1 - 1987/6/5
N2 - A mutant of Escherichia coli with defective Na+/H+ antiporter was isolated. The rationale for its isolation was that cells possessing defective Na+/H+ antiporter, which is essential for establishment of a Na+ gradient, could not grow with a carbon source that was taken up with Na+. The mutant had no appreciable Na+/H+ antiporter activity, but its K+/H+ antiporter and Ca2+/H+ antiporter activities were normal. Judging from the reversion frequency, the defect seems to be due to a single mutation. The mutant could not grow at alkaline pH. Therefore, the Na+/H+ antiporter, but not the K+/H+ antiporter or the Ca2+/H+ antiporter, seems to be responsible for pH regulation in alkaline medium. This mutant will be useful for cloning the Na+/H+ antiporter gene and for detection of Na+-substrate cotransport systems.
AB - A mutant of Escherichia coli with defective Na+/H+ antiporter was isolated. The rationale for its isolation was that cells possessing defective Na+/H+ antiporter, which is essential for establishment of a Na+ gradient, could not grow with a carbon source that was taken up with Na+. The mutant had no appreciable Na+/H+ antiporter activity, but its K+/H+ antiporter and Ca2+/H+ antiporter activities were normal. Judging from the reversion frequency, the defect seems to be due to a single mutation. The mutant could not grow at alkaline pH. Therefore, the Na+/H+ antiporter, but not the K+/H+ antiporter or the Ca2+/H+ antiporter, seems to be responsible for pH regulation in alkaline medium. This mutant will be useful for cloning the Na+/H+ antiporter gene and for detection of Na+-substrate cotransport systems.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023645014&partnerID=8YFLogxK
M3 - Article
C2 - 3034895
AN - SCOPUS:0023645014
VL - 262
SP - 7443
EP - 7446
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 16
ER -