Isolation and physiochemical properties of protein-rich nematode mitochondrial ribosomes

Feng Zhao, Takashi Ohtsuki, Koji Yamada, Shigeo Yoshinari, Kiyoshi Kita, Yoh Ichi Watanabe, Kimitsuna Watanabe

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

In the present study, mitochondrial ribosomes of the nematode Ascaris suum were isolated and their physiochemical properties were compared to ribosomes of Escherichia coli. The sedimentation coefficient and buoyant density of A. suum mitochondrial ribosomes were determined. The sedimentation coefficient of the intact monosome was about 55 S. The buoyant density of formaldehyde-fixed ribosomes in cesium chloride was 1.40 g/cm3, which suggests that the nematode mitoribosomes have a much higher protein composition than other mitoribosomes. The diffusion coefficients obtained from dynamic light scattering measurements were (1.48 ± 0.04) × 10-7 cm2 s-1 for 55 S mitoribosomes and (1.74 ± 0.04) × 10 -7 cm2 s-1 for the 70 S E. coli monosome. The diameter of mitoribosomes was measured by dynamic light-scattering analysis and electron microscopy. Though the nematode mitoribosome has a larger size than the bacterial ribosome, it does not differ significantly in size from mammalian mitoribosomes.

Original languageEnglish
Pages (from-to)9232-9237
Number of pages6
JournalBiochemistry
Volume44
Issue number25
DOIs
Publication statusPublished - Jun 28 2005

ASJC Scopus subject areas

  • Biochemistry

Fingerprint

Dive into the research topics of 'Isolation and physiochemical properties of protein-rich nematode mitochondrial ribosomes'. Together they form a unique fingerprint.

Cite this