Abstract
A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dower 1 × 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 μM added FeCl3. Hydrolysis of the compound yielded alanine, ethanolamine, citric acid and 2-ketoglutaric acid. The 1H-NMR spectrum exhibited the presence of a residue from each of these components in the intact molecule. The fast-atom bombardment mass spectrum of the methyl ester derivative indicated a prominent ion at m/z 477, probably corresponding to [M + 1] ion. Other strains of V. parahaemolyticus were also found to produce this compound when grown in an iron-limited medium.
Original language | English |
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Pages (from-to) | 181-186 |
Number of pages | 6 |
Journal | FEMS Microbiology Letters |
Volume | 94 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - Jul 1 1992 |
Keywords
- Purification
- Siderophore
- Vibrio parahaemolyticus
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics