Isolation and partial characterization of a compound with siderophore activity from Vibrio parahaemolyticus

Shigeo Yamamoto, Yasuhiro Fujita, Noriyuki Okujo, Chiaki Minami, Shyoko Matsuura, Sumio Shinoda

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dower 1 × 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 μM added FeCl3. Hydrolysis of the compound yielded alanine, ethanolamine, citric acid and 2-ketoglutaric acid. The 1H-NMR spectrum exhibited the presence of a residue from each of these components in the intact molecule. The fast-atom bombardment mass spectrum of the methyl ester derivative indicated a prominent ion at m/z 477, probably corresponding to [M + 1] ion. Other strains of V. parahaemolyticus were also found to produce this compound when grown in an iron-limited medium.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalFEMS Microbiology Letters
Volume94
Issue number1-2
DOIs
Publication statusPublished - Jul 1 1992

Keywords

  • Purification
  • Siderophore
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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