Isolation and partial characterization of a compound with siderophore activity from Vibrio parahaemolyticus

Shigeo Yamamoto; Yasuhiro Fujita; Noriyuki Okujo; Chiaki Minami; Shyoko Matsuura; Sumio Shinoda

doi:10.1016/0378-1097(92)90605-N

Isolation and partial characterization of a compound with siderophore activity from Vibrio parahaemolyticus

Shigeo Yamamoto, Yasuhiro Fujita, Noriyuki Okujo, Chiaki Minami, Shyoko Matsuura, Sumio Shinoda

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article

19 Citations (Scopus)

Abstract

A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dower 1 × 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 μM added FeCl₃. Hydrolysis of the compound yielded alanine, ethanolamine, citric acid and 2-ketoglutaric acid. The ¹H-NMR spectrum exhibited the presence of a residue from each of these components in the intact molecule. The fast-atom bombardment mass spectrum of the methyl ester derivative indicated a prominent ion at m/z 477, probably corresponding to [M + 1] ion. Other strains of V. parahaemolyticus were also found to produce this compound when grown in an iron-limited medium.

Original language	English
Pages (from-to)	181-186
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	94
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1097(92)90605-N
Publication status	Published - Jul 1 1992

Keywords

Purification
Siderophore
Vibrio parahaemolyticus

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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Yamamoto, S., Fujita, Y., Okujo, N., Minami, C., Matsuura, S., & Shinoda, S. (1992). Isolation and partial characterization of a compound with siderophore activity from Vibrio parahaemolyticus. FEMS Microbiology Letters, 94(1-2), 181-186. https://doi.org/10.1016/0378-1097(92)90605-N

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AU - Yamamoto, Shigeo

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AU - Minami, Chiaki

AU - Matsuura, Shyoko

AU - Shinoda, Sumio

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