Isolation and partial characterization of a 39 kDa major outer membrane protein of Actinobacillus actinomycetemcomitans Y4

Susumu Kokeguchi, Keijiro Kato, Fusanori Nishimura, Hidemi Kurihara, Yoji Murayama

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The outer membrane fractions of Actinobacillus actinomycetemcomitans, which were extracted from whole cells with cetyl trimethyl ammonium bromide and CaCl2, contained four major outer membrane proteins (MOMP) of 39, 37, 36 and 30 kDa. The 39 kDa MOMP of A. actinomycetemcomitans was sequentially purified by extraction with Zwittergent 3-14 detergent, anion-exchange chromatography and gel filtration chromatography. Analysis of amino acid composition and N-terminal amino acid sequence of 20 residues of purified 39 kDa MOMP was performed. Although some of the periodontitis patient sera reacted strongly with 39 kDa and 30 kDa MOMP in crude outer membrane fractions, purified 39 kDa MOMP showed decreased immunoreactivity with the human sera.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalFEMS Microbiology Letters
Volume77
Issue number1
Publication statusPublished - Jan 1 1991

Fingerprint

Aggregatibacter actinomycetemcomitans
Membrane Proteins
zwittergent 3-14
Membranes
Periodontitis
Serum
Detergents
Gel Chromatography
Anions
Chromatography
Amino Acid Sequence
Amino Acids

Keywords

  • Actinobacillus actinomycetemcomitans
  • Major outer membrane protein (MOMP)

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Isolation and partial characterization of a 39 kDa major outer membrane protein of Actinobacillus actinomycetemcomitans Y4. / Kokeguchi, Susumu; Kato, Keijiro; Nishimura, Fusanori; Kurihara, Hidemi; Murayama, Yoji.

In: FEMS Microbiology Letters, Vol. 77, No. 1, 01.01.1991, p. 85-89.

Research output: Contribution to journalArticle

Kokeguchi, Susumu ; Kato, Keijiro ; Nishimura, Fusanori ; Kurihara, Hidemi ; Murayama, Yoji. / Isolation and partial characterization of a 39 kDa major outer membrane protein of Actinobacillus actinomycetemcomitans Y4. In: FEMS Microbiology Letters. 1991 ; Vol. 77, No. 1. pp. 85-89.
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