Isolation and functional analysis of the gene controlling the stub-spreading trait in rice (Oryza sativa L.)

T. Komori, M. Miyata, Toshio Yamamoto, N. Nitta, Y. Hiei, M. Yano, J. Ueki, T. Komari

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The stub-spreading trait, also designated as 'tiller angle', is one of the components that determine the plant type. In rice, the trait is quite important because of its close relationship to the yield performance. The Spk(ť) gene is a major determinant of the trait in the cross of 'Kasalath' (indica) and 'Nipponbare' (japonica). In this study, the Spk(t) gene was isolated by a map-based cloning strategy. Sequence analysis of the cDNA clones from the locus revealed that the Spk(t) transcripts and the spk(t) transcripts encode identical 259-aa proteins of unknown function; however, the structure of the 3′-untranslated region of each allele is quite different. Additional transgenic experiments in rice demonstrated that the difference is caused by a singlenucleotide polymorphism, at the 3′-splicing site specific to the Spk(i) allele which, thereby plays a critical role in the phenotypic expression. In addition to revealing the molecular mechanism underlying the allele differentiation at the Spk(t) locus, the information presented here should be useful for breeding of this trait in rice.

Original languageEnglish
Pages (from-to)568-575
Number of pages8
JournalPlant Breeding
Volume128
Issue number6
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes

Fingerprint

Oryza sativa
Alleles
alleles
rice
Genes
Plant Structures
loci
genes
3' Untranslated Regions
3' untranslated regions
Breeding
Sequence Analysis
Organism Cloning
molecular cloning
sequence analysis
Complementary DNA
Clone Cells
genetic polymorphism
genetically modified organisms
clones

Keywords

  • Map-based cloning
  • Oryzasativa
  • Stub-spreading

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Isolation and functional analysis of the gene controlling the stub-spreading trait in rice (Oryza sativa L.). / Komori, T.; Miyata, M.; Yamamoto, Toshio; Nitta, N.; Hiei, Y.; Yano, M.; Ueki, J.; Komari, T.

In: Plant Breeding, Vol. 128, No. 6, 01.12.2009, p. 568-575.

Research output: Contribution to journalArticle

Komori, T. ; Miyata, M. ; Yamamoto, Toshio ; Nitta, N. ; Hiei, Y. ; Yano, M. ; Ueki, J. ; Komari, T. / Isolation and functional analysis of the gene controlling the stub-spreading trait in rice (Oryza sativa L.). In: Plant Breeding. 2009 ; Vol. 128, No. 6. pp. 568-575.
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