Isolation and characterization of Ty1/copia-like retrotransposons in mung bean (Vigna radiata)

Weimin Xiao, Yuhui Su, Wataru Sakamoto, Sodmergen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Two Ty1/copia-like retrotransposons, RTvr1 and RTvr2, were isolated from mung bean (Vigna radiata (L.) Wilczek) genomic DNA and are the first complete elements of this kind to be reported in this legume. Nucleotide sequence analyses revealed that both elements are AT-rich (60% and 61%, respectively) and are flanked by a target-site duplication of 5 bp. The structures of RTvr1 and RTvr2 are those of typical long terminal repeat retrotransposons. Both transposons were able to produce putative proteins with the domain order of Gag-protease-integrase-reverse transcriptase-RNase H, indicating that RTvr1 and RTvr2 belong to the Ty1/copia-like retrotransposons. Except for a 2,500-bp insertion region in RTvr2, the overall similarity between RTvr1 and RTvr2 is 92%. Dot blots showed that these two retroelements were present at a copy number of 120 per mung bean haploid genome. Multiple sequence alignments showed that the conserved motifs of the aspartic proteases, integrase, reverse transcriptase, and the RNase H in the Ty1/copia-like group all exist in RTvr1 and RTvr2.

Original languageEnglish
Pages (from-to)323-328
Number of pages6
JournalJournal of Plant Research
Volume120
Issue number2
DOIs
Publication statusPublished - Mar 1 2007

Keywords

  • LTR
  • Mung bean
  • Retrotransposon
  • Reverse transcriptase
  • Ty1/copia

ASJC Scopus subject areas

  • Plant Science

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