Isolation and characterization of two pigment-dispersing hormones from the whiteleg shrimp, Litopenaeus vannamei

Tsuyoshi Ohira, Naoaki Tsutsui, Ichiro Kawazoe, Marcy N. Wilder

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In crustaceans, the pigment-dispersing hormone (PDH) is released from the X-organ/sinus gland complex located in the eyestalks, and controls pigment dispersion in the chromatophores. Knowledge concerning the structure and activity of PDH in penaeid shrimps is remains limited, since natural PDH has been purified from only the Kuruma prawn, Marsupenaeus japonicus. In this study, two PDHs (Liv-PDH-A and -B) were purified from the sinus gland extracts of another penaeid species, the whiteleg shrimp, Litopenaeus vannamei, by two steps of reversed-phase HPLC, and their amino acid sequences were determined. They both consist of 18 amino acid residues, with a free N-terminus and an amidated C-terminus, the sequences of Liv-PDH-A and -B being NSELINSLLGIPKVMNDAamide and NSELINSLLGLPKVMNDAamide, respectively. These sequences are identical to those of mature PDHs deduced from cDNAs encoding L. vannamei PDH precursors cloned previously by other workers. Liv-PDH-A and -B showed significant pigment-dispersing activity in melanophores by in vivo bioassay.

Original languageEnglish
Pages (from-to)601-606
Number of pages6
JournalZoological Science
Volume23
Issue number7
DOIs
Publication statusPublished - Jul 2006
Externally publishedYes

Fingerprint

Litopenaeus vannamei
pigments
hormones
Marsupenaeus japonicus
sinuses
chromatophores
Penaeidae
reversed-phase high performance liquid chromatography
amino acid sequences
bioassays
Crustacea
amino acids
extracts

Keywords

  • Crustacea
  • Eyestalk hormone
  • Litopenaeus vannamei
  • Pigment-dispersing hormone
  • Sinus gland
  • Whiteleg shrimp
  • X-organ

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Isolation and characterization of two pigment-dispersing hormones from the whiteleg shrimp, Litopenaeus vannamei. / Ohira, Tsuyoshi; Tsutsui, Naoaki; Kawazoe, Ichiro; Wilder, Marcy N.

In: Zoological Science, Vol. 23, No. 7, 07.2006, p. 601-606.

Research output: Contribution to journalArticle

Ohira, Tsuyoshi ; Tsutsui, Naoaki ; Kawazoe, Ichiro ; Wilder, Marcy N. / Isolation and characterization of two pigment-dispersing hormones from the whiteleg shrimp, Litopenaeus vannamei. In: Zoological Science. 2006 ; Vol. 23, No. 7. pp. 601-606.
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