Isolation and characterization of the nuclei from Saccharomyces cerevisiae

Kazuo Kamimura, Eiko Tsuchiya, Tokichi Miyakawa, Sakuzo Fuki, Aiko Hirata

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19 Citations (Scopus)

Abstract

We isolated highly intact nuclei from cells of a haploid strain of Saccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalCurrent Microbiology
Volume6
Issue number3
DOIs
Publication statusPublished - 1981
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology

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