Isolation and characterization of temperature-sensitive mutants of the Staphylococcus aureus dnaC gene

Chikara Kaito, Kenji Kurokawa, Muktadir Shahid Hossain, Nobuyoshi Akimitsu, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A protein encoded by the Staphylococcus aureus dnaC gene has 44% and 58% homology with Escherichia coli DnaB and Bacillus subtilis DnaC replicative DNA helicases, respectively. We identified five mutant strains whose temperature-sensitive colony formation phenotypes were complemented by the dnaC gene. DNA replication in these mutants has a fast-stop phenotype, indicating that the S. aureus dnaC gene encodes the replicative DNA helicase required for the elongation step. These mutants were also sensitive to UV irradiation, suggesting that the dnaC gene is involved in DNA repair. The number of viable mutant cells decreased at a non-permissive temperature, suggesting that S. aureus DnaC helicase is a promising target for antibiotics providing bactericidal effects.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalFEMS Microbiology Letters
Volume210
Issue number1
DOIs
Publication statusPublished - Apr 23 2002
Externally publishedYes

Keywords

  • DNA repair
  • DNA replication
  • Staphylococcus aureus
  • Temperature-sensitive mutant
  • dnaC helicase

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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