Isolation and characterization of temperature-sensitive mutants of the Staphylococcus aureus dnaC gene

Chikara Kaito; Kenji Kurokawa; Muktadir Shahid Hossain; Nobuyoshi Akimitsu; Kazuhisa Sekimizu

doi:10.1016/S0378-1097(02)00603-1

Isolation and characterization of temperature-sensitive mutants of the Staphylococcus aureus dnaC gene

Chikara Kaito, Kenji Kurokawa, Muktadir Shahid Hossain, Nobuyoshi Akimitsu, Kazuhisa Sekimizu

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A protein encoded by the Staphylococcus aureus dnaC gene has 44% and 58% homology with Escherichia coli DnaB and Bacillus subtilis DnaC replicative DNA helicases, respectively. We identified five mutant strains whose temperature-sensitive colony formation phenotypes were complemented by the dnaC gene. DNA replication in these mutants has a fast-stop phenotype, indicating that the S. aureus dnaC gene encodes the replicative DNA helicase required for the elongation step. These mutants were also sensitive to UV irradiation, suggesting that the dnaC gene is involved in DNA repair. The number of viable mutant cells decreased at a non-permissive temperature, suggesting that S. aureus DnaC helicase is a promising target for antibiotics providing bactericidal effects.

Original language	English
Pages (from-to)	157-164
Number of pages	8
Journal	FEMS Microbiology Letters
Volume	210
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(02)00603-1
Publication status	Published - Apr 23 2002
Externally published	Yes

Keywords

DNA repair
DNA replication
Staphylococcus aureus
Temperature-sensitive mutant
dnaC helicase

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/S0378-1097(02)00603-1

Cite this

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title = "Isolation and characterization of temperature-sensitive mutants of the Staphylococcus aureus dnaC gene",

abstract = "A protein encoded by the Staphylococcus aureus dnaC gene has 44% and 58% homology with Escherichia coli DnaB and Bacillus subtilis DnaC replicative DNA helicases, respectively. We identified five mutant strains whose temperature-sensitive colony formation phenotypes were complemented by the dnaC gene. DNA replication in these mutants has a fast-stop phenotype, indicating that the S. aureus dnaC gene encodes the replicative DNA helicase required for the elongation step. These mutants were also sensitive to UV irradiation, suggesting that the dnaC gene is involved in DNA repair. The number of viable mutant cells decreased at a non-permissive temperature, suggesting that S. aureus DnaC helicase is a promising target for antibiotics providing bactericidal effects.",

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AU - Kaito, Chikara

AU - Kurokawa, Kenji

AU - Hossain, Muktadir Shahid

AU - Akimitsu, Nobuyoshi

AU - Sekimizu, Kazuhisa

PY - 2002/4/23

Y1 - 2002/4/23

N2 - A protein encoded by the Staphylococcus aureus dnaC gene has 44% and 58% homology with Escherichia coli DnaB and Bacillus subtilis DnaC replicative DNA helicases, respectively. We identified five mutant strains whose temperature-sensitive colony formation phenotypes were complemented by the dnaC gene. DNA replication in these mutants has a fast-stop phenotype, indicating that the S. aureus dnaC gene encodes the replicative DNA helicase required for the elongation step. These mutants were also sensitive to UV irradiation, suggesting that the dnaC gene is involved in DNA repair. The number of viable mutant cells decreased at a non-permissive temperature, suggesting that S. aureus DnaC helicase is a promising target for antibiotics providing bactericidal effects.

AB - A protein encoded by the Staphylococcus aureus dnaC gene has 44% and 58% homology with Escherichia coli DnaB and Bacillus subtilis DnaC replicative DNA helicases, respectively. We identified five mutant strains whose temperature-sensitive colony formation phenotypes were complemented by the dnaC gene. DNA replication in these mutants has a fast-stop phenotype, indicating that the S. aureus dnaC gene encodes the replicative DNA helicase required for the elongation step. These mutants were also sensitive to UV irradiation, suggesting that the dnaC gene is involved in DNA repair. The number of viable mutant cells decreased at a non-permissive temperature, suggesting that S. aureus DnaC helicase is a promising target for antibiotics providing bactericidal effects.

KW - DNA repair

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KW - Temperature-sensitive mutant

KW - dnaC helicase

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Isolation and characterization of temperature-sensitive mutants of the Staphylococcus aureus dnaC gene

Abstract

Keywords

ASJC Scopus subject areas

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